Higher order folding of two different classes of chromatin isolated from chicken erythrocyte nuclei. A light scattering study.
Higher order folding of two different classes of chromatin isolated from chicken erythrocyte nuclei. A light scattering study.
复制标题
从鸡红细胞核中分离出的两种不同类别染色质的高阶折叠。
DOI：
10.1021/bi00534a025
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Bloomfield,VA
中科院分区：
文献类型：
--
作者：
Fulmer,AW;Bloomfield,VA
Andrew W. Fulmer和维克托A.布卢姆菲尔德摘要：用微球菌核酸酶消化鸡红细胞核，切取大量的染色质碎片。通过对含有0.15 M NaCl的缓冲液进行透析，实现S染色质（在生理离子强度下可溶）和I染色质（在生理离子强度下不溶）的分级分离。用动态光散射和静态光散射测定了NaCl浓度对S和I染色质分子尺寸的影响。在离子条件下，通过S和I染色质的凝胶过滤获得一系列片段长度，
Andrew W. Fulmer and Victor A. Bloomfield"'abstract; Bulk chromatinfragments were excised from chicken erythrocyte nuclei by digestion with micrococcal nu-clease. Fractionation into S chromatin (soluble at physiological ionic strengths) and I chromatin (insoluble at physiological ionic strengths) was achieved by dialysis against buffers containing 0.15 M NaCl. The effects of NaCl concentration on the molecular dimensions of S and I chromatins were de-termined by dynamic and static light scattering. Series of fragment lengths were obtained by gel filtration of S and I chromatins under ionic conditions which lead to maximal