Gene rearrangements in lymphoma. Applications to dermatopathology.
Gene rearrangements in lymphoma. Applications to dermatopathology.
复制标题
淋巴瘤中的基因重排。
DOI：
10.1097/00000372-198706000-00006
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Parslow,TG
中科院分区：
文献类型：
--
作者：
LeBoit,PE;Parslow,TG
克隆性被认为是b细胞淋巴瘤的重要诊断特征，但直到最近，t细胞增殖的克隆性还不能直接确定。对淋巴细胞分化中的遗传事件的阐明导致了b细胞和t细胞浸润克隆测定的发展。免疫球蛋白或t细胞受体基因的克隆重排可通过Southern blot技术检测。这种重排提供了克隆增殖的证据，在适当的情况下，可以视为恶性肿瘤的迹象。低分化肿瘤中基因重排的存在可能是造血分化的证据，这种重排的模式可能指向t细胞或b细胞谱系。在皮肤病理学上的具体应用包括评估皮肤淋巴瘤的皮肤活检标本，评估ssamzary综合征的外周血标本，以及评估淋巴结和血液的蕈样真菌病的分期。
Clonality is regarded as an important diagnostic feature of B-cell lymphomas, but until recently the clonality of T-cell proliferations could not be directly determined. Elucidation of the genetic events in lymphocytic differentiation has led to the development of an assay for clonality in both B-and T-cell infiltrates. Clonal rearrangement of the immunoglobulin or T-cell receptor genes can be detected by the Southern blot technique. Such rearrangements provide evidence of clonal proliferation, which, in the proper context, can be regarded as a sign of malignancy. The presence of gene rearrangements in poorly differentiated neoplasms may serve as evidence of hematopoietic differentiation, and the pattern of such rearrangements may point to either T-or B-cell lineage. Specific applications to dermatopathology include the evaluation of skin biopsy specimens for cutaneous lymphoma, evaluation of peripheral blood specimens for detection of Sézary's syndrome, and evaluation of lymph nodes and blood for the staging of mycosis fungoides.