Identification of antioxidant and ACE-inhibitory peptides in fermented milk
Identification of antioxidant and ACE-inhibitory peptides in fermented milk
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DOI：
10.1002/jsfa.2063
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发表时间：
2005-04-30
影响因子：
4.1
通讯作者：
Recio, I
中科院分区：
文献类型：
--
作者：
Hernández-Ledesma, B;Miralles, B;Recio, I
对一种来自欧洲的商业发酵乳的血管紧张素转换酶(ACE)抑制活性和抗氧化性能进行了评价。该乳制品具有中等的ACE抑制活性和ABTS(中心圆点+)自由基清除能力。用反相高效液相色谱(RP-HPLC)从活性最高的组分中提取多肽，用反相高效液相色谱-串联质谱(RP-HPL-MS)进行测序。这项技术可以快速鉴定包括在最有效组分中的多肽，并根据先前的构效关系研究识别各种潜在的活性多肽。已鉴定的序列中有三个以前被描述为有效的ACE抑制剂。一些序列的结构证实了具有ACE抑制、抗氧化和免疫调节活性的多肽的存在。(C)2005年化学工业学会。
The angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant properties of a commercial fermented milk from Europe were evaluated. This dairy product showed moderate ACE-inhibitory activity and ABTS(center dot+) radical-scavenging capacity. The peptides from most active fractions collected by reverse phase high-performance liquid chromatography (RP-HPLC) were sequenced by RP-HPLC-tandem mass spectrometry. This technique allowed rapid identification of peptides included in the most active fractions, and various potentially active peptides were recognised according to previous studies of structure-activity relationship. Three of the identified sequences had previously been described as potent ACE inhibitors. The structure of some sequences substantiated the presence of peptides with ACE-inhibitory, antioxidant and immunomodulatory activities. (c) 2005 Society of Chemical Industry.