Chromatography of Adrenotropic Hormone on Ion-exchange Columns
Chromatography of Adrenotropic Hormone on Ion-exchange Columns
复制标题
促肾上腺皮质激素的离子交换柱色谱分析
作者：
H. Dixon;S. Moore;M. Stack‐Dunne;F. Young
在过去的一年里，有几个观察结果表明，促肾上腺素（ACTH），如抗坏血酸消耗试验1所测量的，可能是一种碱性物质。Payne、拉本和Astwood 2观察到，其活性可以从稀乙酸溶液中吸附到粉末状纤维素上，并再次用0.1 N盐酸洗脱，这些实验与本实验室中关于溶剂分配时促肾上腺素行为的初步试验结果一致（其中一些初步试验是与H. G. Khorana，化学系，剑桥大学）和制备电泳。Astwood、拉本、Payne和Grady 3此后利用氧化纤维素（10.4% COOH）对促肾上腺素活性的单步吸附。同时，我们一直在探索其行为的“Amberlite IEC-50”，这是以前发现允许有效的色谱纯化低分子量的碱性蛋白质，如核糖核酸酶4和溶菌酶5列。
DURING the past year there have been several observations which have indicated that adrenotropic hormone (ACTH), as measured by the ascorbic acid depletion test1, might be a basic substance. Payne, Raben and Astwood2 observed that its activity could be adsorbed on powdered cellulose from dilute acetic acid solution and eluted again by 0.1 N hydrochloric acid, and these experiments agreed with results from preliminary trials in this laboratory on the behaviour of adrenotropic hormone when subjected to solvent partition (some of these initial experiments were carried out in collaboration with Dr. H. G. Khorana, of the Department of Chemistry, University of Cambridge) and preparative electrophoresis. Astwood, Raben, Payne and Grady3 have since utilized a single-step adsorption of adrenotropic hormone activity on oxycellulose (10.4 per cent COOH). We have concurrently been exploring its behaviour on columns of ‘Amberlite IEC-50’, which have previously been found to permit effective chromatographic purification of low-molecular weight basic proteins, such as ribonuclease4 and lysozyme5.