The tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate-signaled defense responses, and glandular trichome development
The tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate-signaled defense responses, and glandular trichome development
复制标题
DOI：
10.1105/tpc.017954
复制
发表时间：
2004-01-01
期刊：
影响因子：
11.6
通讯作者：
Howe, GA
中科院分区：
文献类型：
--
作者：
Li, L;Zhao, YF;Howe, GA
茉莉酸(JA)是一种脂肪酸衍生的信号分子，调节植物对食草动物和一些微生物病原体的广泛防御反应。拟南芥的分子遗传学研究已经证实，JA在花药和花粉发育中也起关键作用，但对植物生活史的其他发育方面不是必需的。在这里，我们描述了一个JA信号缺陷的番茄不育突变体(茉莉酸不敏感1[jai1])的表型和分子特征。虽然突变体表现出花粉活力降低，但不育是由于母体控制种子成熟的缺陷造成的，这与JA调节的蛋白酶抑制蛋白在生殖组织中的积累丢失有关。监狱植物表现出几种与防御相关的表型，包括不能表达JA反应基因，对两点蜘蛛叶的抗性严重受损，以及腺毛的异常发育。我们证明这些缺陷是由番茄CORONATINE-INSENSITIVE1(COI1)同源物功能丧失引起的，COI1是拟南芥中JA信号传导过程所需的一种F-box蛋白。这些发现表明，JA/COI1信号通路在不同植物中调节不同的发育过程，并提示JA在促进腺毛为基础的防御中发挥作用。
Jasmonic acid (JA) is a fatty acid-derived signaling molecule that regulates a broad range of plant defense responses against herbivores and some microbial pathogens. Molecular genetic studies in Arabidopsis have established that JA also performs a critical role in anther and pollen development but is not essential for other developmental aspects of the plant's life cycle. Here, we describe the phenotypic and molecular characterization of a sterile mutant of tomato (jasmonic acid-insensitive1 [jai1]) that is defective in JA signaling. Although the mutant exhibited reduced pollen viability, sterility was caused by a defect in the maternal control of seed maturation, which was associated with the loss of accumulation of JA-regulated proteinase inhibitor proteins in reproductive tissues. jail plants exhibited several defense-related phenotypes, including the inability to express JA-responsive genes, severely compromised resistance to two-spotted spider mites, and abnormal development of glandular trichomes. We demonstrate that these defects are caused by the loss of function of the tomato homolog of CORONATINE-INSENSITIVE1 (COI1), an F-box protein that is required for JA-signaled processes in Arabidopsis. These findings indicate that the JA/COI1 signaling pathway regulates distinct developmental processes in different plants and suggest a role for JA in the promotion of glandular trichome-based defenses.