Boswellia carterii extract inhibits TH1 cytokines and promotes TH2 cytokines in vitro
Boswellia carterii extract inhibits TH1 cytokines and promotes TH2 cytokines in vitro
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DOI：
10.1128/cdli.12.5.575-580.2005
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发表时间：
2005-05-01
期刊：
影响因子：
--
通讯作者：
Via, CS
中科院分区：
文献类型：
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作者：
Chevrier, MR;Ryan, AE;Via, CS
在中国和印度，用于治疗炎症性关节炎的传统草药配方包括博斯韦利亚或锯齿博斯韦利亚。它们都含有乳香酸（BAs），已被证明具有抗炎和抗关节炎的特性。本研究验证了从卡氏杆菌中提取的BAs混合物具有免疫调节特性的假设。采用乙醇提取法制备了产自中国的布氏植物树脂，通过柱层析、高效液相色谱、紫外激光解吸/电离串联质谱等方法对7种BAs的存在进行了确证。然后测试该提取物改变小鼠脾细胞体外产生TH1细胞因子（白细胞介素-2 [IL-2]和γ干扰素）和TH2细胞因子（IL-4和IL-10）的能力。使用乙醇作为溶剂的树脂提取物的递送导致了显著的细胞毒性，而不是单独添加乙醇。相比之下，使用芝麻油溶剂的树脂提取物的递送导致TH1细胞因子的剂量依赖性抑制加上TH2细胞因子的剂量依赖性增强。这些结果表明，纯化后的植物树脂中ba的混合物在体外具有载体依赖性的免疫调节特性。
Traditional herbal formulas used to treat inflammatory arthritis in China and India include Boswellia carterii or Boswellia serrata. They both contain boswellic acids (BAs) which have been shown to exhibit anti-inflammatory and antiarthritic properties. This study tests the hypothesis that mixtures of BAs derived from B. carterii have immunomodulatory properties. B. carterii plant resin obtained from China was prepared as an ethanol extract, and the presence of seven BAs was confirmed by column chromatography, high-performance liquid chromatography, and UV laser desorption/ionization tandem mass spectroscopy. The extract was then tested for its ability to alter in vitro production of TH1 cytokines (interleukin-2 [IL-2] and gamma interferon) and TH2 cytokines (IL-4 and IL-10) by murine splenocytes. Delivery of the resin extract using ethanol as a solvent resulted in significant cellular toxicity not seen with the addition of ethanol alone. By contrast, delivery of the resin extract using a sesame oil solvent resulted in a dose-dependent inhibition of TH1 cytokines coupled with a dose-dependent potentiation of TH2 cytokines. These results indicate that a purified mixture of BAs from B. carterii plant resin exhibits carrier-dependent immunomodulatory properties in vitro.