Overexpression, crystallization and preliminary X-ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis.
Overexpression, crystallization and preliminary X-ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis.
复制标题
结核分枝杆菌磷酸三酯酶的过表达、结晶和初步 X 射线晶体学分析。
DOI：
10.1107/s1744309112049032
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Xin Li
中科院分区：
文献类型：
--
作者：
Liang Zhang;R. Chen;Zhenting Dong;Xin Li
有机磷（OPs）是剧毒化合物，被用作杀虫剂，甚至是化学战剂。磷酸三酯酶（PHPs）通过催化OPs的降解来解毒。迄今为止，已经解决了近100个PHP结构，但结核分枝杆菌（mPHP）的磷酸三酯酶的晶体结构仍然不可用。本研究报道了mPHP的首次结晶。晶体属于C222(1)空间群，晶胞参数a = 68.03, b = 149.60, c = 74.23 Å， α = β = γ = 90°。分析性超离心实验表明，mPHP在溶液中以二聚体的形式存在，即使根据结构数据计算出一个分子存在于不对称单元中。
Organophosphates (OPs) are extremely toxic compounds that are used as insecticides or even as chemical warfare agents. Phosphotriesterases (PHPs) are responsible for the detoxification of OPs by catalysing their degradation. Almost 100 PHP structures have been solved to date, yet the crystal structure of the phosphotriesterase from Mycobacterium tuberculosis (mPHP) remains unavailable. This study reports the first crystallization of mPHP. The crystal belonged to space group C222(1), with unit-cell parameters a = 68.03, b = 149.60, c = 74.23 Å, α = β = γ = 90°. An analytical ultracentrifugation experiment suggested that mPHP exists as a dimer in solution, even though one molecule is calculated to be present in the asymmetric unit according to the structural data.
影响因子：
2.9
作者：
Vanhooke, JL;Benning, MM;Holden, HM
通讯作者：
Holden, HM