Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana
Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana
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DOI：
10.1007/s00425-020-03351-z
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发表时间：
2020-02
期刊：
影响因子：
4.3
通讯作者：
Latifa Nuraini;Yukiko Ando;Kentaro Kawai;F. Tatsuzawa;Kotomi Tanaka;M. Ochiai;Katsumi Suzuki;Verónica Aragonés;J. Daròs;T. Nakatsuka
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文献类型：
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作者：
Latifa Nuraini;Yukiko Ando;Kentaro Kawai;F. Tatsuzawa;Kotomi Tanaka;M. Ochiai;Katsumi Suzuki;Verónica Aragonés;J. Daròs;T. Nakatsuka
主要结论MiMYB 1和MibHLH 2在紫罗兰花色素苷合成中起关键作用。建立了芜菁花叶病毒（Turnip mosaic virus，TMV）的瞬时表达系统。紫罗兰（Matthiola incana（L.）R. Br.）是一种在日本从冬天到春天都很受欢迎的开花植物。在这里，我们观察到，花青苷积累的“复古熏衣草”随着花的发育，而黄酮醇积累保持不变，在整个花的发育。我们获得了5个转录因子基因MiMYB 1、MibHLH 1、MibHLH 2、MiWDR 1和MiWDR 2。酵母双杂交分析显示MiMYB 1与MibHLH 1、MibHLH 2和MiWDR 1相互作用，但MiWDR 2不与任何转录因子相互作用。MiMYB 1和MibHLH 2在花瓣中的表达量随着花芽发育而增加。它们的表达谱与MiF 3 β H、MiDFR、MiANS和Mi 3GT转录本的时间谱以及花青素积累谱具有良好的相关性。而MibHLH 1基因在“熏衣草”各器官中均表达较弱。而在花青苷积累量高于“Vintage Lavender”的品种中，MibHLH 1的表达量较高，而MiWDR 1和MiWDR 2在花发育和营养器官中的表达量基本不变。利用芜菁花叶病毒载体在1个月龄的银莲花幼苗中瞬时表达MiMYB 1，激活了内源花青素生物合成基因MiF 3 β H、MiDFR和MiAN的转录，并诱导了叶片中花青素的异位积累。因此，MiMYB 1可能与MibHLH 2和MiWDR 1相互作用，并且这种三聚体蛋白复合物激活了M. incanflowers中花青素生物合成基因的转录。此外，MibHLH 1与MiMYB 1-MibHLH 2-MiWDR 1复合物一起充当花青素苷生物合成的增强剂。本研究揭示了M.不开花。
Main conclusionMiMYB1andMibHLH2play key roles in anthocyanin biosynthesis inMatthiola incanaflowers. We established a transient expression system using Turnip mosaic virus vector inM. incana.AbstractGarden stock (Matthiola incana(L.) R. Br.) is a popular flowering plant observed from winter to spring in Japan. Here we observed that anthocyanin accumulation in ‘Vintage Lavender’ increased with flower development, whereas flavonol accumulation remained constant throughout flower development. We obtained five transcription factor genes,MiMYB1,MibHLH1,MibHLH2,MiWDR1, andMiWDR2, fromM.incanafloral cDNA contigs. Yeast two-hybrid analyses revealed that MiMYB1 interacted with MibHLH1, MibHLH2, and MiWDR1, but MiWDR2 did not interact with any transcription factor. Expression levels ofMiMYB1andMibHLH2increased in petals during floral bud development. Their expression profiles correlated well with the temporal profiles ofMiF3ʹH,MiDFR,MiANS, andMi3GTtranscripts and anthocyanin accumulation profile. On the other hand,MibHLH1was expressed weakly in all organs of ‘Vintage Lavender’. However, high expression levels ofMibHLH1were detected in petals of other cultivars with higher levels of anthocyanin accumulation than ‘Vintage Lavender’.MiWDR1andMiWDR2maintained constant expression levels in petals during flower development and vegetative organs. TransientMiMYB1expression in 1-month-oldM.incanaseedlings using a Turnip mosaic virus vector activated transcription of the endogenous anthocyanin biosynthetic genesMiF3ʹH,MiDFR, andMiANSand induced ectopic anthocyanin accumulation in leaves. Therefore, MiMYB1 possibly interacts with MibHLH2 and MiWDR1, and this trimeric protein complex activates the transcription of anthocyanin biosynthetic genes inM.incanaflowers. Moreover, MibHLH1 acts as an enhancer of anthocyanin biosynthesis with the MiMYB1–MibHLH2–MiWDR1 complex. This study revealed the molecular mechanism involved in the regulation of anthocyanin accumulation levels inM. incanaflowers.