Fabrication of Bovine Serum Albumin@Au Particles for Colorimetric Detection of Glutathione.
Fabrication of Bovine Serum Albumin@Au Particles for Colorimetric Detection of Glutathione.
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DOI：
10.1021/acsabm.0c01321.s001
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发表时间：
2020-12
影响因子：
4.7
通讯作者：
Xiaoyu Zhang;Zhenzhen Huang;Zilong Guo;Yandong Han;Lijuan Zhang;Wensheng Yang
中科院分区：
文献类型：
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作者：
Xiaoyu Zhang;Zhenzhen Huang;Zilong Guo;Yandong Han;Lijuan Zhang;Wensheng Yang
谷胱甘肽（GSH）的异常浓度是许多人类疾病的重要指标，如癌症、肝损伤、艾滋病和阿尔茨海默病。以110 nm的牛血清白蛋白（BSA）聚集体为模板制备了一种BSA @Au核壳粒子，并通过种子法在其上生长了由Au纳米粒子（NPs）组成的金壳。通过增加生长溶液中含有的银离子的浓度，将沉积在BSA聚集体上的Au壳的形态从Au NPs的稀疏分布调整为密集分布。BSA@Au颗粒的表面等离子体共振（SPR）峰在550至620 nm的范围内是可调谐的，对应于由于壳中的Au NP之间的增强的等离子体耦合而导致的颜色从红色到蓝色的演变。蓝色BSA@Au颗粒经鉴定可用于GSH的比色检测，因为GSH可通过破坏BSA聚集体中的分子间二硫键而作为BSA@Au颗粒的溶胀剂。随着GSH含量的增加，BSA@Au颗粒的颜色从蓝色逐渐变为红色，这是由于BSA@Au颗粒逐渐溶胀，从而增加了壳层中Au纳米颗粒之间的距离，这很容易被肉眼识别或通过紫外-可见（UV-vis）光谱记录。该比色法用于真实的样品中GSH的分析，具有良好的选择性和抗干扰能力。此外，我们还设计并制备了一种用于GSH检测的固体传感系统，将BSA@Au颗粒分散在琼脂糖凝胶中。
Abnormal concentrations of glutathione (GSH) are important indicators of many human diseases such as cancers, liver damage, AIDS, and Alzheimer's disease. In this work, a kind of bovine serum albumin (BSA)@Au core-shell particles were fabricated using 110 nm BSA aggregates as a template, onto which gold shells composed of Au nanoparticles (NPs) were grown through a seeded growth approach. The morphology of the Au shells deposited on BSA aggregates was tuned from sparse to dense distribution of Au NPs by increasing the concentration of silver ions contained in the growth solutions. Surface plasmon resonance (SPR) peaks of BSA@Au particles were tunable in the range from 550 to 620 nm, corresponding to evolution in color from red to blue due to the enhanced plasmonic coupling among the Au NPs in the shell. The blue BSA@Au particles were qualified for colorimetric detection of GSH since GSH may act as a swelling agent for BSA@Au particles by breaking the intermolecular disulfide bonds in BSA aggregates. With an increased amount of GSH presented, the color of BSA@Au particles evolved from blue to red attributed to gradual swelling of BSA@Au particles and thus increased the distance among the Au NPs in the shell, which was readily recognized by naked eyes or recorded by ultraviolet-visible (UV-vis) spectroscopy. This colorimetric method exhibited good selectivity and anti-interference capability in the analysis of GSH in real samples. In addition, a solid sensing system for the detection of GSH was designed and fabricated by dispersing BSA@Au particles into an agarose hydrogel.