Cloning and Characterization of karmoisin Homologue Gene （Nlka） in Two Brown Planthopper Strains with Different Eye Colors
Cloning and Characterization of karmoisin Homologue Gene （Nlka） in Two Brown Planthopper Strains with Different Eye Colors
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DOI：
10.1016/j.rsci.2016.02.005
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发表时间：
2016-03
期刊：
影响因子：
4.8
通讯作者：
Shu-Hua Liu;Tang Jian;L. Ju;Bao-jun Yang;Aiying Wang;Jin‐cai Wu
中科院分区：
文献类型：
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作者：
Shu-Hua Liu;Tang Jian;L. Ju;Bao-jun Yang;Aiying Wang;Jin‐cai Wu
褐飞虱(BPH)是亚洲水稻的一种毁灭性害虫。与褐眼色野生型不同，褐飞虱还具有红眼色突变表型。作为一种可见的遗传标记，褐飞虱红眼突变体是一种有价值的材料。为了揭示BPH的眼色突变机制，从BPH中克隆了Akarmoisin同源基因(命名为asNlka)。在其他昆虫中，Andkarmoisin一直被认为是与黄褐素相关的基因，编码苯恶嗪酮合成酶(PHS)，Nlkais由7个外显子组成，编码502个氨基酸(NlKA)。NlKA与昆虫同源物具有较高的氨基酸同源性(48.8%~51.8%)，在所有发育阶段和所测试的所有组织中均可检测到NlkA转录本，包括卵、若虫、成虫、体壁、卵巢、脂肪体、中肠和马氏管。然而，突变株和野生型菌株之间没有观察到恒定的In/Del或非同义突变。实时定量聚合酶链式反应实验也表明，它们之间的转录水平没有显著差异。这些结果表明，Nlka1不是引起BPH红眼突变表型的靶基因。通过二级结构和基序分析，本研究还表明，所有从昆虫的甘油三烯中推导出的蛋白质可能都是单羧酸转运体(MCTs)的成员，而不是PHSs。
The brown planthopper (BPH),Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic marker, the red-eye mutant in BPH is a valuable material. To reveal the eye color mutation mechanism, akarmoisinhomologue gene (named asNlka) was cloned from BPH. Andkarmoisinis always deemd as a xanthommatin-related gene in other insects, encoding phenoxazinone synthetase (PHS).Nlkais consisted of 7 exons and encodes a protein with 502 amino acids (NlKA). NlKA showed high amino acid identities with its insect homologues (48.8%–51.8%).Nlkatranscripts can be detected at all the developmental stages and in all tissues tested, including egg, nymph, adult, body wall, ovary, fat body, midgut and Malpighian tubule. However, no constant In/Del or non-synonymous mutation was observed between the mutant and the wild type strains. Quantitative real-time PCR experiment also showed thatNlkatranscript level had no significant differences between them. These results indicated thatNlkais not the target gene causing the red-eye color mutation phenotype of BPH. Through the second structure and motif analysis, the present study also showed that all the proteins deduced from thekarmoisingenes in insects may be members of monocarboxylate transporters (MCTs) rather than PHSs.