Expression of the Epidermal Growth Factor System in Eutopic Endometrium from Women with Endometriosis Differs from That in Endometrium from Healthy Women
Expression of the Epidermal Growth Factor System in Eutopic Endometrium from Women with Endometriosis Differs from That in Endometrium from Healthy Women
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DOI：
10.1159/000167798
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发表时间：
2009-01-01
影响因子：
2.1
通讯作者：
Nexo, Ebba
中科院分区：
文献类型：
--
作者：
Ejskjaer, Kirsten;Sorensen, Boe Sandahl;Nexo, Ebba
背景资料：表皮生长因子（EGF）系统包括四种受体、HER 1 -4和几种配体，并且在健康生育妇女的子宫内膜中周期性表达。我们的目的是确定表皮生长因子系统之间的差异表达子宫内膜异位症和正常子宫内膜。方法：我们以前检查EGF系统从健康妇女（n = 14）的子宫内膜标本。在这里，我们检查了子宫内膜（n = 23），腺瘤（n = 10）和腹膜子宫内膜异位症（n = 9）的子宫内膜异位症妇女（n = 23）的样本。通过实时PCR分析EGF系统的受体和配体的mRNA表达，并通过免疫组织化学定位蛋白质。结果：与我们以前在健康子宫内膜中的发现相比，子宫内膜中HER 1 -3的mRNA较高，而HER 4和配体则无变化。子宫内膜异位瘤显示较低的HER 1 -3表达和无HER 4表达。与健康女性相比，HER 1和HER 2在晚期分泌期以及HER 3在增殖期表现出显著差异。免疫组织化学，HER 2被确定在所有样品中，主要是在腺体和表面上皮。在少数腺体中，HER 2既存在于细胞质中，也存在于细胞膜中。结论：我们报告定量和定性差异EGF系统在异位在位子宫内膜相比，子宫内膜从健康的个人。版权所有（C）2008 S. Karger AG，巴塞尔
Background: The epidermal growth factor (EGF) system comprises four receptors, HER1-4, and several ligands, and is cyclically expressed in endometrium from healthy fertile women. Our aim is to identify differences in expression of the EGF system between endometriotic and normal endometrium. Methods: We previously examined the EGF system in endometrial samples from healthy women (n = 14). Here we examine samples from endometrium (n = 23), endometriomas (n = 10) and peritoneal endometriosis (n = 9) from women with endometriosis (n = 23). mRNA expression of receptors and ligands from the EGF system was analyzed by real-time PCR, and proteins were localized by immunohistochemistry. Results: Endometrial mRNA for HER1-3 was high compared with our previous findings in healthy endometrium, whereas HER4 and the ligands were unchanged. Endometriomas show lower expression of HER1-3 and no HER4 expression. Significant differences were demonstrated in late secretory phase for HER1 and HER2 and in the proliferative phase for HER3 compared to healthy women. Immunohistochemically, HER2 was identified in all samples, predominately in glands and surface epithelium. In a few glands, HER2 was in both cytoplasm and cell membrane. Conclusion: We report quantitative and qualitative differences in the EGF system in endometriotic eutopic endometrium compared to endometrium from healthy individuals. Copyright (C) 2008 S. Karger AG, Basel