High throughput label-free platform for statistical bio-molecular sensing
High throughput label-free platform for statistical bio-molecular sensing
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DOI：
10.1039/c1lc20116f
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Boisen, Anja
中科院分区：
文献类型：
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作者：
Bosco, Filippo G.;Hwu, En-Te;Boisen, Anja
传感器在许多日常操作中至关重要，包括安全、环境控制、人体诊断和患者监测。筛选和在线监测需要可靠和高通量的传感。我们报告的演示的高通量无标记的传感器平台，利用悬臂梁传感器。这些传感器经常被称赞为促进高度并行化的操作。不幸的是，到目前为止，还没有提出提供大数据集以及容易的液体样品处理的概念。我们使用DVD播放器的光学和机械来处理液体样品，并读出悬臂梁偏转和谐振频率。此外，测量表面粗糙度。当结合悬臂梁偏转的粗糙度被发现持有有价值的额外信息的特异性和非特异性结合事件。在几分钟内，可以并行分析30个液体样品，每个样品由24个基于MEMS的传感器进行分析。该方法用于检测链霉亲和素与抗体的结合。
Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.