In vivo dedifferentiation of human epidermal cells
In vivo dedifferentiation of human epidermal cells
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DOI：
10.1016/j.cellbi.2007.05.016
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发表时间：
2007-11-01
影响因子：
3.9
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Li, Haihong;Fu, Xiaobing;Wang, Jun
与我们之前的研究一致，我们在此提供了进一步的证据来证明表皮细胞在体内去分化分化为干细胞或类干细胞。将去除基底细胞的表皮片用6-二脒基-2-苯基吲哚(DAPI)标记，然后移植到裸鼠全层皮肤伤口处。存活片的免疫组织化学检查显示，移植后第 7 天，一些细胞在棘层和颗粒层中 DAPI 和细胞角蛋白（CK19、CK14）或 β1 整合素均呈阳性。此外，通过流式细胞术测定，与异种移植前相比，移植后 7 d 存活表皮片移植物中 α 6(bri) CDdim 和 α 6(bri) CD71(bri) 群体的百分比显着增加（P < 0.05）。结果共同表明，移植表皮片中的一些分化细胞在体内去分化为干细胞或类干细胞，这为我们提供了去分化的新证据和见解。 (c) 2007 年由 Elsevier Ltd 代表国际细胞生物学联合会出版。
Consistent with our previous study, we herein offer further evidence to demonstrate the dedifferentiation of differentiating epidermal cells into stem cells or stem cells-like in vivo. The epidermal sheets eliminated of basal cells were labeled with 6-diamidino-2-phenylindole (DAPI), and then were transplanted onto the full-thickness skin wounds nude mice. lmmunohistochemical examination of the survival sheets showed that some cells were positive for both DAPI and either cytokeratins (CK19, CK14) or beta 1 integrin in spinous and granular layers at day 7 after transplantation. Furthermore, there was a significant increase in the percentages of both alpha 6(bri) CDdim and alpha 6(bri) CD71(bri) populations in survival epidermal sheet grafts 7 d after transplantation compared with those before xenotransplantation (P < 0.05), as determined by flow cytometry. The results collectively indicated that some of the differentiated cells in engrafted epidermal sheets dedifferentiated into stem cells or stem cellslike in vivo, which offer us new evidence and insights into the dedifferentiation. (c) 2007 Published by Elsevier Ltd on behalf of International Federation for Cell Biology.