A transient increase in histone H2A ubiquitination is coincident with the onset of erythroleukemic cell differentiation
A transient increase in histone H2A ubiquitination is coincident with the onset of erythroleukemic cell differentiation
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组蛋白 H2A 泛素化的短暂增加与红白血病细胞分化的开始同时发生
DOI：
10.1182/blood.v71.4.1153.bloodjournal7141153
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发表时间：
1988
期刊：
影响因子：
20.3
通讯作者：
D. Housman
中科院分区：
文献类型：
--
作者：
J. Hensold;P. Swerdlow;D. Housman
小鼠红白血病细胞可用于研究红系分化的调节，因为当用各种诱导剂处理时，这些恶性原成红细胞分化为正染性成红细胞。染色质蛋白的变化已被描述诱导剂暴露后。这些变化在终末分化细胞中最大，其意义尚不清楚。泛素是一种高度保守的8.5千道尔顿肽，与高达10%的组蛋白H2A共价连接。我们证明，MEL细胞暴露于分化诱导剂后，H2A的泛素化发生短暂增加。这种变化与分化的开始是一致的。这一结果表明，泛素化的H2A可能有必要的红白血病细胞分化的核变化的作用。
Murine erythroleukemia cells are useful for studying the regulation of erythroid differentiation since these malignant pronormoblasts differentiate to orthochromatic normoblasts when treated with a variety of inducing agents. Changes in chromatin proteins have been described following inducer exposure. The significance of these changes, which are greatest in terminally differentiated cells remains unknown. Ubiquitin is a highly conserved 8.5 kilodalton peptide that is covalently linked to up to 10% of histone H2A. We demonstrate that following exposure of MEL cells to inducers of differentiation, a transient increase in ubiquitination of H2A occurs. This change is coincident with the onset of differentiation. This result suggests that ubiquitination of H2A may have a role in the nuclear changes necessary for erythroleukemic cell differentiation.