Effect of 2, 4-epibrassinolide on main health-promoting compounds in broccoli sprouts
Effect of 2, 4-epibrassinolide on main health-promoting compounds in broccoli sprouts
复制标题
DOI：
10.1016/j.lwt.2014.02.047
复制
发表时间：
2014-09-01
影响因子：
6
通讯作者：
Wang, Qiaomei
中科院分区：
文献类型：
--
作者：
Guo, Rongfang;Hou, Qiumei;Wang, Qiaomei
研究了2，4-表油菜素（EBR）（2、20、200 nmol/L）和NaCl（0、40、160 mmol/L）对青花菜芽萌发率、鲜重、抗坏血酸和硫代葡萄糖苷含量、黑芥子酶活性及抗氧化能力的影响。EBR对青花菜芽中硫代葡萄糖苷含量的影响因EBR和NaCl浓度不同而异。20 nmol/L EBR加40 mmol/L NaCl处理西兰花芽，总硫代葡萄糖甙和萝卜苷含量分别增加86%和85%，黑芥子酶活性在加或不加NaCl的情况下均得到显著提高。20 nmol/L EBR处理后，抗坏血酸含量显著增加，且20 nmol/L EBR +40 mmol/L NaCl联合处理比40 mmol/L NaCl单独处理显著促进了抗坏血酸的积累。这些结果表明，2nmol/L EBR +40 mmol/LNaCl组合处理以及20 nmol/L EBR单独处理都可以选择性地提高西兰花芽中主要的健康促进物质硫代葡萄糖苷和抗坏血酸的含量。(C)2014爱思唯尔有限公司版权所有。
The combined treatment with 2, 4-epibrassinolide (EBR) (2, 20, and 200 nmol/L) and NaCl (0, 40, and 160 mmol/L) on the germination rate, fresh weight, contents of ascorbic acid and glucosinolates, activities of myrosinase and antioxidant ability of broccoli sprouts was investigated. The influence of EBR on the content of glucosinolates in broccoli sprouts varied with the concentrations of EBR and NaCl. The content of total glucosinolates and glucoraphanin in broccoli sprouts treated with 2 nmol/L EBR plus 40 mmol/L NaCl was increased by 86% and 85%, respectively, and the activity of myrosinase was significantly improved by 20 nmol/L EBR treatment with or without NaCl. In addition, the content of ascorbic acid was significantly increased after treatment with 20 nmol/L EBR, and the combined treatment with 20 nmol/L EBR plus 40 mmol/L NaCl significantly enhanced the accumulation of ascorbic acid when compared with the 40 mmol/L NaCl treatment alone. These results indicated that the combined treatment with 2 nmol/L EBR plus 40 mmol/L NaCl as well as EBR treatment solely at 20 nmol/L could be useful ways for selective increase of glucosinolates or ascorbic acid, the main health-promoting compounds in broccoli sprouts. (C) 2014 Elsevier Ltd. All rights reserved.