Transcriptional analysis of in vivo Plasmodium yoelii liver stage gene expression
Transcriptional analysis of in vivo Plasmodium yoelii liver stage gene expression
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DOI：
10.1016/j.molbiopara.2005.03.018
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发表时间：
2005-08-01
影响因子：
1.5
通讯作者：
Aguiar, JC
中科院分区：
文献类型：
--
作者：
Sacci, JB;Ribeiro, JMC;Aguiar, JC
疟原虫体内肝脏阶段的转录库在很大程度上仍然是未知的，似乎不适合传统的分子分析，因为少量的寄生虫和大量的未感染的肝细胞。我们已经克服了这个障碍，利用激光捕获显微切割提供了一个高质量的来源，寄生虫的mRNA构建的肝脏阶段的cDNA文库。该库的测序和注释表明，约氏疟原虫在肝细胞发育过程中表达了623种不同的基因。在这些基因中，25%似乎是肝脏阶段所特有的。这是第一次对约氏疟原虫肝脏阶段的体内基因表达进行全面分析，并提供了对约氏疟原虫基因在发育的这一关键阶段差异表达的见解。(c)2005 Elsevier B.V.保留所有权利。
The transcriptional repertoire of the in vivo liver stage of Plasmodium has remained largely unidentified and seemingly not amenable to traditional molecular analysis because of the small number of parasites and large number of uninfected hepatocytes. We have overcome this obstruction by utilizing laser capture microdissection to provide a high quality source of parasite mRNA for the construction of a liver stage cDNA library. Sequencing and annotation of this library demonstrated expression of 623 different Plasmodium yoelii genes during development in the hepatocyte. Of these genes, 25% appear to be unique to the liver stage. This is the first comprehensive analysis of in vivo gene expression undertaken for the liver stage of P. yoelii, and provides insights into the differential expression of P. yoelii genes during this critical stage of development. (c) 2005 Elsevier B.V. All rights reserved.