Drosophila liprin-α and the receptor phosphatase Dlar control synapse morphogenesis
Drosophila liprin-α and the receptor phosphatase Dlar control synapse morphogenesis
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DOI：
10.1016/s0896-6273(02)00643-8
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发表时间：
2002-03-28
期刊：
影响因子：
16.2
通讯作者：
Van Vactor, D
中科院分区：
文献类型：
--
作者：
Kaufmann, N;DeProto, J;Van Vactor, D
在这里，我们研究的受体蛋白酪氨酸磷酸酶（RPTP），Dlar，和相关的细胞内蛋白质，Dliprin-alpha，在果蝇幼虫神经肌肉接头的突触功能。我们发现，Dliprin-alpha和Dlar是正常的突触形态所需的。我们还发现突触的复杂性与Dlar基因产物的量成正比，这表明Dlar活性决定突触的大小。超微结构分析表明，Dliprin-alpha和Dlar需要定义突触前活动区的大小和形状。因此，在两种突变体中突触传递都伴随减少。最后，上位性分析表明Dliprin-alpha是Dlar在突触上的作用所必需的。这些数据表明一个模型，其中Dliprin-alpha和Dlar合作调节突触前蛋白网络的形成和/或维持。
Here, we examine the synaptic function of the receptor protein tyrosine phosphatase (RPTP), Dlar, and an associated intracellular protein, Dliprin-alpha, at the Drosophila larval neuromuscular junction. We show that Dliprin-alpha and Dlar are required for normal synaptic morphology. We also find that synapse complexity is proportional to the amount of Dlar gene product, suggesting that Dlar activity determines synapse size. Ultrastructural analysis reveals that Dliprin-alpha and Dlar are required to define the size and shape of the presynaptic active zone. Accordingly, there is a concomitant decrease in synaptic transmission in both mutants. Finally, epistasis analysis indicates that Dliprin-alpha is required for Dlar's action at the synapse. These data suggest a model where Dliprin-alpha and Dlar cooperate to regulate the formation and/or maintenance of a network of presynaptic proteins.