Nitric oxide is significantly reduced in ex vivo porcine arteries during reverse flow because of increased superoxide production
Nitric oxide is significantly reduced in ex vivo porcine arteries during reverse flow because of increased superoxide production
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DOI：
10.1113/jphysiol.2004.075218
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发表时间：
2004-12-01
影响因子：
5.5
通讯作者：
Kassab, GS
中科院分区：
文献类型：
--
作者：
Lu, X;Kassab, GS
振荡和负流通常发生在心血管系统中，这使这些区域容易发生动脉粥样硬化。一氧化氮（NO）的产生与血流大小成比例增加，并被认为是动脉粥样硬化的保护。然而，尚不清楚的是流动逆转对NO浓度（[NO]）的影响。本研究的假设是[NO]在逆向流动中减少。另一个假设是，在回流过程中，[NO]的减少是通过超氧化物产生的增加来介导的。这些假设在猪弹性动脉和肌肉动脉的离体制备中得到了验证。生理溶液在容器中的流动以正向和反向方向进行调节，并使用重氮偶联法和高效液相色谱相结合的方法检测流出物的亚硝酸盐水平。结果表明，在回流过程中，[NO]明显减少。此外，加入tempol（超氧化物歧化酶模拟物），这是一种超氧化物清除剂，在逆向流动中返回[NO]，以反映正向流动。这些结果具有重要意义，因为超氧化物的作用与许多心血管疾病有关，目前的发现表明，血流逆转应该被添加到列表中。
Oscillatory and negative flows occur normally in the cardiovascular system, which predispose those regions to atherosclerosis. Nitric oxide (NO) production increases in proportion to the magnitude of flow and is known to be athero-protective. What is not known, however, is the effect of flow reversal on NO concentration ([NO]). The hypothesis of the present study is that [NO] is reduced in reverse flow. An additional hypothesis is that the reduction in [NO] is mediated through an increase in superoxide production during flow reversal. These hypotheses were tested in an ex vivo preparation of porcine elastic and muscular arteries. The flow of a physiological solution through the vessels was regulated in the forward and reverse direction and the effluent was assayed for nitrite levels using a combination of a diazo coupling method and high performance liquid chromatography. Our results show that [NO] is significantly reduced during reverse flow. Furthermore, addition of tempol (superoxide dismutase-mimetic) which is a superoxide scavenger returns the [NO] during reverse flow to mirror those of forward flow. These results have important implications since the action of superoxide is implicated in many cardiovascular diseases, and the present finding suggests that flow reversal should be added to the list.