Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length
Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length
复制标题
纤毛蛋白 2、7 和 9 以及 MAP4 沿初级纤毛的轴丝集聚并控制纤毛的长度
DOI：
10.1242/jcs.111377
复制
发表时间：
2013-06-15
影响因子：
4
通讯作者：
Benmerah, Alexandre
中科院分区：
文献类型：
--
作者：
Ghossoub, Rania;Hu, Qicong;Benmerah, Alexandre
Septins是一个大的、进化上保守的GTP酶家族，其形成异源寡聚体并与基于肌动蛋白的细胞骨架和微管相互作用。它们参与支架功能，并在芽殖酵母、精子鞭毛和肾上皮细胞的初级纤毛基底中形成扩散屏障。我们研究了septins在视网膜色素上皮（RPE）细胞的初级纤毛中的作用，发现SEPT2与SEPT7和SEPT9形成1：1：1的复合物，并且该复合物的三个成员沿轴丝的长度共定位沿着。与肾上皮细胞中的观察结果相似，通过基于siRNA的方法消耗纤毛定位的septins抑制了纤毛发生。MAP4是SEPT2的结合伴侣并控制隔膜蛋白对微管的可及性，也定位于轴丝，在那里它似乎负调节纤毛长度。总之，我们的数据提供了新的见解septins和MAP4的功能和调节的组织中的初级纤毛和微管为基础的活动在细胞中。
Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton and microtubules. They are involved in scaffolding functions, and form diffusion barriers in budding yeast, the sperm flagellum and the base of primary cilia of kidney epithelial cells. We investigated the role of septins in the primary cilium of retinal pigmented epithelial (RPE) cells, and found that SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9 and that the three members of this complex colocalize along the length of the axoneme. Similar to observations in kidney epithelial cells, depletion of cilium-localized septins by siRNA-based approaches inhibited ciliogenesis. MAP4, which is a binding partner of SEPT2 and controls the accessibility of septins to microtubules, was also localized to the axoneme where it appeared to negatively regulate ciliary length. Taken together, our data provide new insights into the functions and regulation of septins and MAP4 in the organization of the primary cilium and microtubule-based activities in cells.