Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq.
Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq.
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DOI：
10.7554/elife.30560
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发表时间：
2017-12-12
期刊：
影响因子：
7.7
通讯作者：
Chang M
中科院分区：
文献类型：
--
作者：
Claussin C;Porubský D;Spierings DC;Halsema N;Rentas S;Guryev V;Lansdorp PM;Chang M
涉及姐妹染色单体的同源重组是重组介导的 DNA 修复中最准确、也是最常用的形式。尽管姐妹染色单体重组很重要，但它并不容易研究，因为它不会导致 DNA 序列发生变化，使得姐妹染色单体之间的重组难以检测。我们之前开发了一种新型 DNA 模板链测序技术，称为 Strand-seq，可用于绘制单细胞全基因组范围内的姐妹染色单体交换 (SCE) 事件。 SCE 率的增加是重组活性升高和基因组不稳定的指标，这是癌症的标志。在本研究中，我们采用 Strand-seq 来检测酿酒酵母中的 SCE。我们提供了第一个可量化的证据，证明野生型细胞中大多数自发的 SCE 事件并不是由于 DNA 双链断裂的修复所致。
Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. We provide the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks.