Identification of a Novel Class of Target Genes and a Novel Type of Binding Sequence of Heat Shock Transcription Factor in Saccharomyces cerevisiae*
Identification of a Novel Class of Target Genes and a Novel Type of Binding Sequence of Heat Shock Transcription Factor in Saccharomyces cerevisiae*
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DOI：
10.1074/jbc.m411256200
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发表时间：
2005-03
影响因子：
4.8
通讯作者：
A. Yamamoto;Y. Mizukami;H. Sakurai
中科院分区：
文献类型：
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作者：
A. Yamamoto;Y. Mizukami;H. Sakurai
热休克转录因子（HSF）激活一组编码热休克蛋白（HSPs）的基因的转录。HSP基因的启动子区含有称为热休克元件（HSE）的HSF结合序列，其由序列5′-nGAAn-3′（其中n是任何核苷酸）的连续反向重复序列组成。我们构建了酿酒酵母hsf 1突变体，并分析了突变细胞中热休克反应的全基因组变化。结果表明，Hsf 1不仅对HSP的热诱导转录是必需的，而且对编码参与不同细胞过程的蛋白质的基因也是必需的，所述过程包括蛋白质降解、解毒、能量产生、碳水化合物代谢和维持细胞壁完整性。大约一半的Hsf 1调控基因在其启动子区域缺乏典型的HSE。相反，这些基因中的几个具有新的Hsf 1结合序列，其包含三个被5 bp中断的nTTCn（或nGAAn）的直接重复。重复单元的数量和间距是热诱导转录以及Hsf 1识别的关键决定因素。在酵母基因组中，该序列的存在富含Hsf 1调节基因，表明它通常用作Hsf 1调节子中的HSE。
In response to hyperthermia, heat shock transcription factor (HSF) activates transcription of a set of genes encoding heat shock proteins (HSPs). The promoter regions of HSP genes contain the HSF binding sequence called the heat shock element (HSE), which consists of contiguous inverted repeats of the sequence 5′-nGAAn-3′ (where n is any nucleotide). We have constructed an hsf1 mutant of Saccharomyces cerevisiae and analyzed genome-wide changes in heat shock response in the mutant cells. The results have revealed that Hsf1 is necessary for heat-induced transcription of not only HSP but also genes encoding proteins involved in diverse cellular processes such as protein degradation, detoxification, energy generation, carbohydrate metabolism, and maintenance of cell wall integrity. Approximately half of the Hsf1-regulated genes lacked the typical HSE in their promoter regions. Instead, several of these genes have a novel Hsf1 binding sequence that contains three direct repeats of nTTCn (or nGAAn) interrupted by 5 bp. The number and spacing of the repeating units are critical determinants for heat-induced transcription as well as for recognition by Hsf1. In the yeast genome, the presence of the sequence is enriched in Hsf1-regulated genes, suggesting that it is generally used as an HSE in the Hsf1 regulon.