Utility of cytokeratin 7 for distinguishing chromophobe renal cell carcinoma from renal oncocytoma
Utility of cytokeratin 7 for distinguishing chromophobe renal cell carcinoma from renal oncocytoma
复制标题
DOI：
10.1159/000020172
复制
发表时间：
2000-04-01
期刊：
影响因子：
23.4
通讯作者：
Gosselin, B
中科院分区：
文献类型：
--
作者：
Leroy, X;Moukassa, D;Gosselin, B
目的：嫌色细胞肾癌是一种相对少见的肾癌，1985年报道。主要的鉴别诊断是肾嗜酸细胞瘤。黑尔胶体铁染色是形态学解释的有力辅助手段，但它不具有特异性，有时难以解释。为探讨细胞角蛋白7在肾嫌色细胞癌与肾嗜酸细胞瘤鉴别诊断中的价值，采用免疫组化方法，对6例肾嫌色细胞癌和11例肾嗜酸细胞瘤的石蜡包埋组织进行细胞角蛋白7的免疫组化染色结果：所有嫌色细胞肾细胞癌均表现出强烈的细胞质染色，周围细胞加重。与此相反，11例嗜酸细胞瘤中有8例完全阴性，3例仅在小于5%的肿瘤细胞中显示弱和局灶性染色。结论：当黑尔胶体铁染色不确定时，细胞角蛋白7免疫组化染色可用于肾嗜酸细胞瘤和嫌色细胞癌的鉴别诊断。版权所有（C）2000 S. Karger AG，巴塞尔。
Objective: Chromophobe renal cell carcinoma is a relatively uncommon variant of renal carcinoma described in 1985. The main differential diagnosis is renal oncocytoma. Hale's colloidal iron staining is a powerful adjunct to morphological interpretation but it is not specific and is sometimes difficult to interpret. We studied the immunohistochemical expression of cytokeratin 7 to determine its value in distinguishing chromophobe renal cell carcinoma from renal oncocytoma.Methods: Immunostaining was performed on paraffin-embedded tumor tissue of 6 chromophobe renal cell carcinomas and 11 oncocytomas with an antibody to cytokeratin 7 (clone OVTL 12/30, Dako, France) using a streptavidin-biotin method.Results: All chromophobe renal cell carcinomas showed strong cytoplasmic staining with peripheral cell accentuation. In contrast, 8 of 11 oncocytomas were entirely negative and 3 showed only weak and focal staining in less than 5% of the tumor cells.Conclusion: Immunohistochemical staining for cytokeratin 7 may be useful for the differential diagnosis of renal oncocytomas and chromophobe renal cell carcinomas when Hale's colloidal iron staining is uncertain. Copyright (C) 2000 S. Karger AG, Basel.