Rewiring of Afferent Fibers in the Somatosensory Thalamus of Mice Caused by Peripheral Sensory Nerve Transection
Rewiring of Afferent Fibers in the Somatosensory Thalamus of Mice Caused by Peripheral Sensory Nerve Transection
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DOI：
10.1523/jneurosci.5008-11.2012
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发表时间：
2012-05-16
影响因子：
5.3
通讯作者：
Miyata, Mariko
中科院分区：
文献类型：
--
作者：
Takeuchi, Yuichi;Yamasaki, Miwako;Miyata, Mariko
周围感觉神经损伤后神经回路的重塑和突触功效的变化被认为是脑功能重组的基础，包括感受野的变化。然而，何时或如何重塑发生在很大程度上是未知的。在这里，我们显示了快速重新布线的传入纤维在成熟的腹后内侧丘脑核的小鼠周围触须感觉神经横断后，使用全细胞电压钳技术。横断诱导的传入纤维的招聘丘脑中继神经元损伤后5-6天内。重布线是通路特异性的，但不依赖于感觉经验或周围神经活动。新招募的纤维介导的小EPSC，和突触后含谷氨酸2的AMPA受体选择性上调在新的突触。丘脑回路的这种快速和路径特异性重塑可能是在脊髓上水平的大规模轴突重组的第一步，这发生在外周感觉神经损伤后数月或数年。
The remodeling of neural circuitry and changes in synaptic efficacy after peripheral sensory nerve injury are considered the basis for functional reorganization in the brain, including changes in receptive fields. However, when or how the remodeling occurs is largely unknown. Here we show the rapid rewiring of afferent fibers in the mature ventral posteromedial thalamic nucleus of mice after transection of the peripheral whisker sensory nerve, using the whole-cell voltage-clamp technique. Transection induced the recruitment of afferent fibers to a thalamic relay neuron within 5-6 d of injury. The rewiring was pathway specific, but not sensory experience dependent or peripheral nerve activity dependent. The newly recruited fibers mediated small EPSCs, and postsynaptic GluA2-containing AMPA receptors were selectively upregulated at the new synapses. This rapid and pathway-specific remodeling of thalamic circuitry may be an initial step in the massive axonal reorganization at supraspinal levels, which occurs months or years after peripheral sensory nerve injury.