IL3-DEPENDENT MOUSE CLONES THAT EXPRESS B-220 SURFACE-ANTIGEN, CONTAIN IG GENES IN GERM-LINE CONFIGURATION, AND GENERATE LYMPHOCYTES-B INVIVO
IL3-DEPENDENT MOUSE CLONES THAT EXPRESS B-220 SURFACE-ANTIGEN, CONTAIN IG GENES IN GERM-LINE CONFIGURATION, AND GENERATE LYMPHOCYTES-B INVIVO
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DOI：
10.1016/s0092-8674(85)80053-2
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
STEINMETZ, M
中科院分区：
文献类型：
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作者：
PALACIOS, R;STEINMETZ, M
从MRL/LPR、Cba/J和BALB/c小鼠的骨髓中建立了L/BAgA2、Cb/BM7、BA/C1和Bc/BM11四个连续增殖系。这些克隆携带B细胞系表面抗原B-220，但不携带通常在成熟B淋巴细胞、髓细胞或T淋巴细胞上表达的抗原。他们的ig.MU。H链和.kappa。L链基因处于胚系构型。G418[O-2-amino-2，7-dideoxy-.alpha.-D-glycero-D-glycoheptopyranosyl-(1.fwdarw。4)-O-[3-deoxy-4-C-methyl-3-methylamino-.beta.-L-arabinopyranosyl-(1.fwdarw。用逆转录病毒载体将[-2-脱氧-D-链胺]抗性基因导入每个克隆中，作为转基因细胞后代的选择性标记。克隆株L/BAgA2、CB/BM7和BC/BM11在体内移植后可发育为抗体分泌细胞，而不能发育为抗体分泌细胞。没有一种细胞对多克隆T细胞激活剂有反应，也没有任何细胞在体外分化为可以发育为粒/巨噬细胞集落的细胞。它们都生长在白介素3中，但不生长在其他细胞因子中。L/BAgA2、CB/BM7和BC/BM11克隆是B淋巴细胞的早期前体。
The continuously proliferating clones L/B AgA2, CB/Bm 7, Ba/C1 and Bc/Bm 11 were established from bone marrow of MRL/LPR, CBA/J and BALB/c mice. These clones carry the B cell lineage surface antigen B-220 but not antigens normally expressed on mature B lymphocytes, myeloid cells, or T lymphocytes. Their Ig .mu. H chain and .kappa. L chain genes are in germ-line configuration. The G418 [O-2-amino-2,7-dideoxy-.alpha.-D-glycero-D-glycoheptopyranosyl-(1 .fwdarw. 4)-O-[3-deoxy-4-C-methyl-3-methylamino-.beta.-L-arabinopyranosyl-(1 .fwdarw. 6)]-2-deoxy-D-streptamine] resistance gene was introduced into each clone with a retrovirus vector and then used as a selective marker for the progeny of transfected cells. Clones L/B AgA2, CB/Bm 7 and Bc/Bm 11, but not Ba/C1, could develop into antibody-secreting cells after in vivo transfer. None gave rise to cells responsive to polyclonal T cell activators, nor did any differentiate into cells that could develop into granulocyte/macrophage-colony-forming cells in vitro. All grew in interleukin 3 but not in other cytokines. Clones L/B AgA2, CB/Bm 7 and Bc/Bm 11 are early precursors of B lymphocytes.