Monocytes recruited into the alveolar air space of mice show a monocytic phenotype but upregulate CD14
Monocytes recruited into the alveolar air space of mice show a monocytic phenotype but upregulate CD14
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DOI：
10.1152/ajplung.2001.280.1.l58
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发表时间：
2001-01-01
影响因子：
4.9
通讯作者：
Lohmeyer, J
中科院分区：
文献类型：
--
作者：
Maus, U;Herold, S;Lohmeyer, J
在生理和炎症条件下，单核细胞被招募到肺泡空间的评估受到了这些细胞与常驻肺泡巨噬细胞（rAMs）区分困难的阻碍。利用在公羊中积累而不标记血液白细胞的静脉注射荧光染料PKH26，我们开发了一种技术，可以对小鼠肺泡中募集的单核细胞进行鉴定、分离和功能分析。鼠脑乙脑是人单核细胞化学引诱蛋白(MCP)-1 （JE/MCP-1）的同系物，在小鼠肺泡沉积引起单核细胞内流，这些单核细胞通过支气管肺泡灌洗恢复，并通过流式细胞术从pkh26染色的公羊中分离出来。通过细胞表面F4/80、CD11a、CD11b、CD18、CD49d和CD62L的表达评估，肺泡募集的单核细胞表现为血液单核细胞表型。相比之下，CD14在肺泡募集的单核细胞上明显上调，同时肿瘤坏死因子- α信息增加，将这种单核细胞群与外周血单核细胞和rAMs区分开来。因此，在对乙脑/MCP-1的反应中，被募集到小鼠肺泡空间的单核细胞保持了血液单核细胞的表型特征，但上调了CD14，并“启动”了对内毒素的反应，增加了细胞因子的表达。
The evaluation of monocytes recruited into the alveolar space under both physiological and inflammatory conditions is hampered by difficulties in discriminating these cells from resident alveolar macrophages (rAMs). Using the intravenous injected fluorescent dye PKH26, which accumulated in rAMs without labeling blood leukocytes, we developed a technique that permits the identification, isolation, and functional analysis of monocytes recruited into lung alveoli of mice. Alveolar deposition of murine JE, the homologue of human monocyte chemoattractant protein (MCP)-1 (JE/MCP-1), in mice provoked an alveolar influx of monocytes that were recovered by bronchoalveolar lavage and separated from PKH26-stained rAMs by flow cytometry. Alveolar recruited monocytes showed a blood monocytic phenotype as assessed by cell surface expression of F4/80, CD11a, CD11b, CD18, CD49d, and CD62L. In contrast, CD14 was markedly upregulated on alveolar recruited monocytes together with increased tumor necrosis factor-alpha message, discriminating this monocyte population from peripheral blood monocytes and rAMs. Thus monocytes recruited into the alveolar air space of mice in response to JE/MCP-1 keep phenotypic features of blood monocytes but upregulate CD14 and are "primed" for enhanced responsiveness to endotoxin with increased cytokine expression.