Dental biofilms at healthy and inflamed gingival margins
Dental biofilms at healthy and inflamed gingival margins
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DOI：
10.1034/j.1600-051x.2002.290609.x
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发表时间：
2002-06-01
影响因子：
6.7
通讯作者：
Olsson, J
中科院分区：
文献类型：
--
作者：
Rüdiger, SG;Carlén, A;Olsson, J
目的：牙龈炎症中观察到的菌斑形成增加尚不完全清楚。牙菌膜中的受体蛋白可能影响细菌的粘附和随后的菌斑形成。本研究的目的是检查蛋白质和微生物在牙齿生物膜，在健康和发炎的牙龈margins.Material和方法：看看边缘炎症是否会影响组成的表膜和早期牙菌斑，样品取自牙龈和切部的牙齿牙周健康的受试者，无论是在牙龈健康和实验性牙龈炎。采用凝胶电泳、免疫印迹和图像分析对膜蛋白进行分析。结果：牙龈炎时，菌斑形成率明显增加，菌斑形成率随时间延长而增加。蛋白质的半定量量和细菌的数量在个体和表面之间变化很大。然而，牙龈炎期间总的和单个的表膜蛋白的量以及细菌的总数增加，并且在牙齿表面的切缘部分上蛋白质的增加具有统计学显著性。与健康牙龈相比，放线菌属的数量减少。（切部只）和链球菌和牙周病原体的数量增加，在4小时的牙生物膜被认为是在发炎gingiva.Conclusion：增加龈沟液流量牙龈炎影响表膜的形成和增加血浆蛋白在表膜可能修改细菌附着和早期牙菌斑组成。
Objectives: The increased plaque formation observed in gingival inflammation is not fully understood. Receptor proteins in the dental pellicle might influence bacterial adhesion and subsequent plaque formation. The purpose of the present study was to examine proteins and microorganisms in dental biofilms, at healthy and inflamed gingival margins.Material and methods: To see whether marginal inflammation affects the composition of the pellicle and the early dental plaque, samples were taken from the gingival and incisal parts of teeth in periodontally healthy subjects, both in gingival health and during experimental gingivitis. Pellicle proteins were analysed using gel-electrophoresis, immunoblotting and image analysis. Bacteria were analysed by culturing and the PCR technique.Results: During gingivitis, the rate of plaque formation increased significantly. The semiquantitative amounts of proteins and the numbers of bacteria varied considerably between individuals and surfaces. The amount of total and individual pellicle proteins and the total numbers of bacteria were, however, increased during gingivitis and the increase in proteins was statistically significant on the incisal parts of tooth surfaces. Compared to a healthy gingiva, reduced numbers for Actinomyces spp. (incisal parts only) and streptococci and increased numbers of periodontopathogens in the 4 h dental biofilms were seen at the inflamed gingiva.Conclusion: Increased gingival crevicular fluid flow during gingivitis affects pellicle formation and increased plasma proteins in the pellicle may modify bacterial attachment and early dental plaque composition.