Atomic Force Microscopy of DNA and DNA-Protein Interactions.
Atomic Force Microscopy of DNA and DNA-Protein Interactions.
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DOI：
10.1007/978-1-0716-2221-6_5
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发表时间：
2022
影响因子：
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通讯作者：
Phil Haynes;Kavit H. S. Main;Bernice Akpinar;Alice L. B. Pyne
中科院分区：
文献类型：
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作者：
Phil Haynes;Kavit H. S. Main;Bernice Akpinar;Alice L. B. Pyne
原子力显微镜（AFM）是一种显微镜技术，使用锋利的探针以纳米分辨率跟踪样品表面。对于生物应用，其关键优势之一是能够在水环境中可视化单个分子和分子复合物的子结构。在这里，我们描述了应用原子力显微镜来确定二级和三级结构的表面结合的DNA，其与蛋白质的相互作用。
Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometer resolution. For biological applications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins.