IDENTIFICATION OF LIPOXIN-A4 AND ITS RELATIONSHIP TO THE SULFIDOPEPTIDE LEUKOTRIENE-C4, LEUKOTRIENE-D4, AND LEUKOTRIENE-E4 IN THE BRONCHOALVEOLAR LAVAGE FLUIDS OBTAINED FROM PATIENTS WITH SELECTED PULMONARY-DISEASES
IDENTIFICATION OF LIPOXIN-A4 AND ITS RELATIONSHIP TO THE SULFIDOPEPTIDE LEUKOTRIENE-C4, LEUKOTRIENE-D4, AND LEUKOTRIENE-E4 IN THE BRONCHOALVEOLAR LAVAGE FLUIDS OBTAINED FROM PATIENTS WITH SELECTED PULMONARY-DISEASES
复制标题
DOI：
10.1164/ajrccm/141.6.1453
复制
发表时间：
1990-06-01
期刊：
影响因子：
--
通讯作者：
SERHAN, CN
中科院分区：
文献类型：
--
作者：
LEE, TH;CREA, AEG;SERHAN, CN
脂氧素是具有生物活性的含有三羟基四烯的产物，源自花生四烯酸，是通过脂氧合酶之间的相互作用形成的。尽管脂氧素是在体外从混合细胞悬浮液中产生的，但尚未确定这些产品是否在体内合成。我们对 12 名肺部疾病患者（结节病，6 名；肺炎，2 名；哮喘，2 名；癌症，1 名；原因不明的肺泡炎，1 名）和 6 名正常对照受试者进行了支气管肺泡灌洗 (BAL)。使用气相色谱质谱法和选择性离子监测分析 BAL 液体中的脂氧素 A4 (LXA4)，并使用反相高效液相色谱和放射免疫测定法测定硫肽白三烯的水平。在所研究的 12 名患者中，有 9 名患者的 BAL 液中检测到了 LXA4。 LXA4 的水平范围为 0.4 至 2.8 ng/ml。在六名正常受试者中均未检测到 LXA4。所有BAL样本中均检测到硫肽白三烯，含量范围为0.04至0.7 ng/ml，患者与正常人之间无显着差异。在BAL液中可检测到LXA4的患者中，LXA4与硫肽白三烯的浓度之比范围为1.9至62（平均值为19.0）。这是 LXA4 在疾病中存在的首次证明。
Lipoxins are biologically active trihydroxytetraene containing products derived from arachidonic acid that are formed by interactions between lipoxygenases. Although the lipoxins have been generated from mixed cell suspensions in vitro, it was not been established whether these products are synthesized in vivo. We have performed bronchoalveolar lavage (BAL) in 12 patients with lung disease (sarcoid, six; pneumonia, two; asthma, two; carcinoma, one; alveolitis of unknown cause, one) and in six normal control subjects. The BAL fluid was analyzed for lipoxin A4 (LXA4) using gas chromatography mass spectrometry with selective ion monitoring, and the levels of the sulfidopeptide leukotrienes were determined using reverse-phase high-performance liquid chromatography and radioimmunoassay. LXA4 was detected in BAL fluid from nine of the 12 patients studied. The levels of LXA4 ranged from 0.4 to 2.8 ng/ml. LXA4 was not detected in any of the six normal subjects. Sulfidopeptide leukotrienes were detected in all the BAL samples, ranging from 0.04 to 0.7 ng/ml, and there was no significant difference between the patients and the normal subjects. In patients with detectable LXA4 in BAL fluid, the ratio of the concentrations of LXA4 to those of the sulfidopeptide leukotrienes ranged from 1.9 to 62 (mean, 19.0). This is the first demonstration of the presence of LXA4 in disease.