Identification of Macrophage Inhibitory Cytokine-1 in Adipose Tissue and Its Secretion as an Adipokine by Human Adipocytes
Identification of Macrophage Inhibitory Cytokine-1 in Adipose Tissue and Its Secretion as an Adipokine by Human Adipocytes
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DOI：
10.1210/en.2008-0952
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发表时间：
2009-04-01
期刊：
影响因子：
4.8
通讯作者：
Bing, Chen
中科院分区：
文献类型：
--
作者：
Ding, Qi;Mracek, Tomas;Bing, Chen
巨噬细胞抑制性细胞因子-1（MIC-1）是TGF-β超家族的一个分支成员，参与多种细胞过程的控制，并通过抑制食欲介导恶病质。脂肪组织作为一种内分泌器官，分泌调节能量平衡和其他细胞功能的蛋白质（脂肪因子）。本研究调查了MIC-1是否在脂肪组织中表达以及MIC-1是否是脂肪细胞的分泌产物。从不同的仓库收集小鼠和人脂肪组织。在细胞培养中诱导3 T3-L1前脂肪细胞和人前脂肪细胞分化为脂肪细胞。MIC-1 mRNA在小鼠主要脂肪库（附睾、肾周、皮下）中检测到。在这些储库中，MIC-1基因表达在分离的成熟脂肪细胞和基质血管细胞中是明显的。在3 T3-L1脂肪细胞中，分化前后均检测到MIC-1 mRNA。MIC-1 mRNA和蛋白质分泌在人前脂肪细胞以及分化的脂肪细胞中是明显的。H2 O2和15 d-前列腺素J刺激人脂肪细胞产生MIC-1（2）。此外，重组MIC-1增加分化的人脂肪细胞的脂联素分泌。在人皮下和内脏脂肪中也观察到MIC-1 mRNA和蛋白。MIC-1 mRNA水平与脂联素mRNA水平呈正相关。此外，MIC-1 mRNA与人体体重指数和体脂量呈负相关。我们的结论是MIC-1在脂肪组织中表达并由脂肪细胞分泌，因此是一种新的脂肪因子。MIC-1可能在调节脂肪组织功能和体脂量中具有旁分泌作用。（内分泌学150：1688-1696，2009）
Macrophage inhibitory cytokine-1 (MIC-1), a divergent member of the TGF-beta superfamily, is involved in the control of multiple cellular processes and mediates cachexia through the inhibition of appetite. Adipose tissue as an endocrine organ secretes proteins (adipokines) that regulate energy homeostasis and other cellular functions. This study investigated whether MIC-1 is expressed in adipose tissue and whether MIC-1 is a secretory product of adipocytes. Mouse and human adipose tissues were collected from different depots. 3T3-L1 preadipocytes and human preadipocytes were induced to differentiate into adipocytes in cell culture. MIC-1 mRNA was detected in the major mouse adipose depots (epididymal, perirenal, sc). In these depots, MIC-1 gene expression was evident in both isolated mature adipocytes and stromal-vascular cells. In 3T3-L1 adipocytes, MIC-1 mRNA was detected before and after differentiation. MIC-1 mRNA and protein secretion were evident in human preadipocytes as well as differentiated adipocytes. MIC-1 production by human adipocytes was stimulated by H2O2 and 15d-prostaglandin J(2). In addition, recombinant MIC-1 increased adiponectin secretion by differentiated human adipocytes. MIC-1 mRNA and protein were also observed in human sc and visceral fat. MIC-1 mRNA levels were positively correlated with adiponectin mRNA. Moreover, MIC-1 mRNA was negatively associated with body mass index and body fat mass in human subjects. We conclude that MIC-1 is expressed in adipose tissue and secreted from adipocytes and is therefore a new adipokine. MIC-1 may have a paracrine role in the modulation of adipose tissue function and body fat mass. (Endocrinology 150: 1688-1696, 2009)