A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties
A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties
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DOI：
10.1007/s10123-020-00147-9
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发表时间：
2020-10-29
影响因子：
3.1
通讯作者：
Hosseini, Seyedeh-Roya
中科院分区：
文献类型：
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作者：
Ashengroph, Morahem;Hosseini, Seyedeh-Roya
本研究的目的是分离和表征海洋细菌菌株能够将亚硒酸盐转化为元素硒，形成硒纳米颗粒（SeNPs）。首次从里海沿岸分离到一株属于解淀粉芽孢杆菌（Bacillus amyloliquefaciens）的新型海洋菌株（GenBank登录号MK 392020），并基于其在有氧条件下将亚硒酸盐转化为SeNPs的能力进行表征。通过颜色变化证实SeNP的初步形成，并通过UV-Vis光谱表征产物。场发射扫描电子显微镜（FESEM）与能量色散X-射线（EDX）分析表明，在细菌生物质的表面和上清液中的球形SeNPs的存在。动态光散射（DLS）分析显示SeNP具有约45.4- 68.3nm的平均粒度（Z-平均）。X射线衍射（XRD）研究证实了生物合成的SeNPs的无定形性质。傅里叶变换红外光谱（FTIR）的研究表明，典型的蛋白质和脂质相关的带作为封端剂的SeNPs。不同的有效参数对应于B的SeNPs产量。在静息细胞策略下对解淀粉杆菌菌株SRB 04进行了优化。结果表明，最佳工艺条件为：亚硒酸根含氧阴离子浓度为2 mM，菌体量为20 g/L，反应时间为60 h。合成的纳米硒对金黄色葡萄球菌具有显著的抗菌活性，与氯霉素相比，氯霉素是一种广谱抗生素。
The aim of this study was to isolate and characterize marine bacterial strains capable of converting selenite to elemental selenium with the formation of Se nanoparticles (SeNPs). For the first time, a novel marine strain belonging to Bacillus amyloliquefaciens (GenBank accession no. MK392020) was isolated from the coast of the Caspian Sea and characterized based on its ability for transformation of selenite to SeNPs under aerobic conditions. The preliminary formation of SeNPs was confirmed via color changes and the products characterized by UV-Vis spectroscopy. The field-emission scanning electron microscopy (FESEM) together with energy-dispersive X-ray (EDX) analysis showed the presence of the spherical SeNPs on both the surface of the bacterial biomass and in the supernatant solution. Dynamic light scattering (DLS) analysis showed the SeNPs to have an average particle size (Z-average) around 45.4-68.3 nm. The X-ray diffraction (XRD) studies substantiated the amorphous nature of the biosynthesized SeNPs. Fourier-transform infrared spectroscopic (FTIR) studies of the SeNPs indicated typical proteinaceous and lipid-related bands as capping agents on the SeNPs. Different effective parameters corresponding the yield of SeNPs by B. amyloliquefaciens strain SRB04 were optimized under resting cell strategy. Results showed that the optimal process conditions for SeNP production were 2 mM of selenite oxyanion, 20 g/L of cell biomass, and 60 h reaction time. The synthesized SeNPs had a remarkable antibacterial activity on Staphylococcus aureus compared with chloramphenicol as a broad-spectrum antibiotic.