In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens
In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens
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DOI：
10.1016/j.vaccine.2021.08.104
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发表时间：
2021-09-22
期刊：
影响因子：
5.5
通讯作者：
Ohashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Fujisawa, Sotaro;Murata, Shiro;Ohashi, Kazuhiko
家禽红螨（Dermanyssus gallinae; PRM）是一种吸血的鸡体外寄生虫，对全世界的家禽养殖业构成威胁，并显着降低产蛋业的生产力。在家禽养殖场中广泛用于预防PRM的化学杀螨剂由于杀螨剂抗性PRM的出现而经常无效。因此，需要替代的控制方法，并且疫苗接种是控制PRM的有希望的策略。根据先前的RNA测序分析，一种新的脂肪细胞质膜相关蛋白样分子（Dg-APMAP）在血液喂养的PRM中高度表达。在这里，我们试图确定DgAPMAP的全序列，研究其在不同生命阶段的PRM中的表达，并评估其作为疫苗抗原的潜力。Dg-APMAP mRNA表达在中肠和卵巢，并在所有生命阶段，无论喂养状态。重要的是，在体外饲养的PRM与血浆来源于鸡免疫的重组蛋白的胞外区的Dg-APMAP显着降低了它们的存活率在哺乳动物和成人，这需要血餐。我们的数据表明，Dg-APMAP疫苗接种诱导的宿主免疫应答可能是减少家禽业中PRM引起的痛苦的有效策略。(c)2021爱思唯尔有限公司版权所有。
The poultry red mite (Dermanyssus gallinae; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of DgAPMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry. (c) 2021 Elsevier Ltd. All rights reserved.