Adipose tissue expression of the glycerol channel aquaporin-7 gene is altered in severe obesity but not in type 2 diabetes
Adipose tissue expression of the glycerol channel aquaporin-7 gene is altered in severe obesity but not in type 2 diabetes
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DOI：
10.1210/jc.2007-0531
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发表时间：
2007-09-01
影响因子：
5.8
通讯作者：
Vendrell, J.
中科院分区：
文献类型：
--
作者：
Ceperuelo-Mallafre, V.;Miranda, M.;Vendrell, J.
内容：Aquaporin-7是必需的从脂肪细胞的甘油流出和影响全身葡萄糖稳态在animal studies. Objective：我们的目的是测试的假设，AQP7基因表达水平可能会受到肥胖和2型糖尿病在human.Design：肥胖研究队列包括12瘦，22 nonsevere肥胖，和13严重肥胖的科目。2型糖尿病研究队列包括17名瘦型和39名肥胖型2型糖尿病患者。测定血浆可溶性蛋白单核细胞趋化蛋白-1、TNF受体1和2、IL-6和甘油的循环水平。通过实时PCR测量皮下脂肪组织AQP7、MCP-1、IL-6、TNF α、PPAR γ和SREBP1c基因的基因表达。结果：重度肥胖组AQP7基因表达水平低于精瘦组和非重度肥胖组（P <0.001）。此外，循环甘油浓度在严重肥胖的受试者中较低，但没有观察到与AQP7脂肪组织表达相关。AQP7表达与促炎基因呈负相关（单核细胞趋化蛋白-1，r =-0.203和P = 0.044; TNF α，r =-0.209和P = 0.036）。在成脂因子方面，AQP7表达水平与PPAR γ mRNA表达水平呈正相关（r = 0.265; P = 0.012）。结论：AQP7在女性重度肥胖患者中表达下调。这种甘油通道的表达不受2型糖尿病的影响。
Context: Aquaporin-7 is required for efflux of glycerol from adipocytes and influences whole-body glucose homeostasis in animal studies.Objective: Our objective was to test the hypothesis that AQP7 gene expression levels may be affected by presence of obesity and type 2 diabetes in humans.Design: The obesity study cohort consisted of 12 lean, 22 nonseverely obese, and 13 severely obese subjects. The type 2 diabetes study cohort consisted of 17 lean and 39 obese type 2 diabetic patients. Circulating levels of plasma soluble proteins monocyte chemoattractant protein-1, TNF receptors 1 and 2, and IL-6 and glycerol were measured. The sc adipose tissue gene expression of AQP7, MCP-1, IL-6, TNF alpha, PPAR gamma, and SREBP1c genes was measured by real-time PCR. AQP7 gene mutation analysis was performed.Results: Severely obese women showed lower AQP7 expression levels compared with lean and nonseverely obese ( P < 0.001). Moreover, circulating glycerol concentration was lower in severely obese subjects, but no correlation with AQP7 adipose tissue expression was observed. AQP7 expression was negatively related with proinflammatory genes ( for monocyte chemoattractant protein-1, r = -0.203 and P = 0.044; for TNF alpha, r = -0.209 and P = 0.036). Concerning adipogenic factors, AQP7 expression levels were found to be positively determined by PPAR gamma mRNAexpression levels ( r = 0.265; P = 0.012). AQP7 expression did not show differences regarding the presence of type 2 diabetes.Conclusion: Expression of AQP7 is down-regulated in women with severe obesity. The expression of this glycerol channel is not affected by type 2 diabetes.