Non-Invasive Optical Guided Tumor Metastasis/Vessel Imaging by Using Lanthanide Nanoprobe with Enhanced Down-Shifting Emission beyond 1500 nm
Non-Invasive Optical Guided Tumor Metastasis/Vessel Imaging by Using Lanthanide Nanoprobe with Enhanced Down-Shifting Emission beyond 1500 nm
复制标题
使用增强型下移发射超过 1500 nm 的镧系元素纳米探针进行非侵入性光引导肿瘤转移/血管成像
DOI：
10.1021/acsnano.8b05431
复制
发表时间：
2019-01-01
期刊：
影响因子：
17.1
通讯作者：
Hao, Jianhua
中科院分区：
文献类型：
--
作者：
Li, Youbin;Zeng, Songjun;Hao, Jianhua
肿瘤血管/转移和脑血管结构的可视化对分析脑疾病的病理状态和肿瘤血管异常，提高肿瘤诊断水平具有重要意义。利用1500 nm以上的二次近红外发射进行体内荧光成像（NIR-IIb）是一种新一代光学成像方法，在成像灵敏度和空间分辨率方面都有显著提高。不幸的是，一种能够产生足够亮度和均匀尺寸的NIR-IIb发射的高度生物相容性探针仍然稀缺。在这里，我们提出了聚丙烯酸（PAA）修饰的NaLnF(4):40Gd/20Yb/2Er纳米棒（Ln = Y, Yb, Lu, PAA-Ln- nrs）具有增强的降移NIR-IIb发射，高量子产率（QY），相对较窄的带宽（类似于160 nm），以及通过Ce3+掺杂的高生物相容性，用于高性能NIR-IIb生物成像。通过Ce3+掺杂，在抑制Y、Yb和Lu主体上转换（UC）路径的同时，1500 nm以上的降移发射提高了1.75-2.2倍。此外，与传统使用的基载体相比，钌基探针在水中NIR-IIb发射的QY从2.2%提高到3.6%。探索的明亮NIR-IIb发射的paa - lu - nr用于高灵敏度小肿瘤（约4 mm）/转移性微小肿瘤（约3 mm）检测，高空间分辨率肿瘤血管可视化（41 μ m）和脑血管成像。因此，我们的研究结果为利用基于镧系元素的NIR-IIb探针进行体内光学引导肿瘤血管/转移和无创脑血管成像开辟了机会。
Visualization of tumor vessels/metastasis and cerebrovascular architecture is vitally important for analyzing pathological states of brain diseases and a tumor's abnormal blood vessels to improve cancer diagnoses. In vivo fluorescence imaging using second near-infrared emission beyond 1500 nm (NIR-IIb) has emerged as a next generation optical imaging method with significant improvement in imaging sensitivity and spatial resolution. Unfortunately, a highly biocompatible probe capable of generating NIR-IIb emission with sufficient brightness and uniformed size is still scarce. Here, we have proposed the poly(acrylic acid) (PAA)-modified NaLnF(4):40Gd/20Yb/2Er nanorods (Ln = Y, Yb, Lu, PAA-Ln-NRs) with enhanced downshifting NIR-IIb emission, high quantum yield (QY), relatively narrow bandwidth (similar to 160 nm), and high biocompatibility via Ce3+ doping for high performance NIR-IIb bioimaging. The downshifting emission beyond 1500 nm is improved by 1.75-2.2 times with simultaneously suppressing the upconversion (UC) path in Y, Yb, and Lu hosts via Ce3+ doping. Moreover, compared with the traditionally used Y-based host, the QY of NIR-IIb emission in the Lu-based probe in water is improved from 2.2% to 3.6%. The explored bright NIR-IIb emitted PAA-Lu-NRs were used for high sensitivity small tumor (similar to 4 mm)/metastatic tiny tumor detection (similar to 3 mm), tumor vessel visualization with high spatial resolution (41 mu m), and brain vessel imaging. Therefore, our findings open up the opportunity of utilizing the lanthanide based NIR-IIb probe with bright 1525 nm emission for in vivo optical-guided tumor vessel/metastasis and noninvasive brain vascular imaging.