Quantitative analysis of iridoids, secoiridoids, xanthones and xanthone glycosides in Gentiana lutea L. roots by RP-HPLC and LC-MS
Quantitative analysis of iridoids, secoiridoids, xanthones and xanthone glycosides in Gentiana lutea L. roots by RP-HPLC and LC-MS
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DOI：
10.1016/j.jpba.2007.07.001
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发表时间：
2007-11-05
影响因子：
3.4
通讯作者：
Ganzera, Markus
中科院分区：
文献类型：
--
作者：
Aberham, Anita;Schwaiger, Stefan;Ganzera, Markus
本文描述的高效液相色谱方法能够测定龙胆根中所有主要的、目前已知的生物活性化合物。在RP-18色谱柱上，以0.025%三氟乙酸水溶液、乙腈和正丙醇为流动相，可以分离环烯醚萜类化合物(马钱酸)、二环烯醚萜类化合物(苦参苦苷、龙胆苦苷、苋菜素、苦瓜苷)、黄原酮类化合物(龙胆素、异龙胆素)和两种黄酮苷类化合物(龙胆皂苷)。该方法是敏感的(LOD
The here described HPLC-method enables the determination of all major, currently known bioactive compounds in gentian roots. A separation of iridoids (loganic acid), secoiridoids (swertiamarin, gentiopicroside, amarogentin, sweroside), xanthones (gentisin, isogentisin) and two xanthone glycosides (gentiosides) was possible on RP-18 column material, using 0.025% aqueous TFA, acetonitrile and n-propanol as mobile phase. The method is sensitive (LOD