Changes in high endothelial venules in lymph nodes after vascularized and nonvascularized lymph node transfer in a murine autograft model
Changes in high endothelial venules in lymph nodes after vascularized and nonvascularized lymph node transfer in a murine autograft model
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DOI：
10.1002/jso.25365
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发表时间：
2019-05-01
影响因子：
2.5
通讯作者：
Yamamoto, Yuhei
中科院分区：
文献类型：
--
作者：
Ishikawa, Kosuke;Funayama, Emi;Yamamoto, Yuhei
背景和目的血管化淋巴结转移（LNT）在淋巴水肿的治疗中越来越受欢迎。然而，目前尚不清楚转移淋巴结的血管化是否有助于功能改善。高内皮小静脉（HEVs）是允许淋巴细胞进入LNs的特殊血管。在这项研究中，我们比较了血管化和非血管化LNT后LNs中hev和淋巴细胞的数量。方法50只小鼠随机分为3组（1组带蒂带血管化LNT； 2组带蒂无血管化LNT； 3组游离无血管化LNT）。正蓝染色证实传入淋巴再连接。术后4周切除转移的ln。对hev、b细胞和t细胞进行免疫组化染色和定量。结果1组20例转移淋巴结中有13例出现传入淋巴重新连接，2组15例中有11例，3组15例中有7例。传入淋巴重新连接的转移LNs中，1组扩张/总hev的比率显著高于2和3组。b细胞和t细胞数量在转移的LNs中无显著差异。结论血管化LNT术后传入淋巴再连接成功的hev比非血管化LNT术后保留的hev更多。
Background and objectives Vascularized lymph node transfer (LNT) is gaining popularity in the treatment of lymphedema. However, it is unclear whether the vascularization of transferred lymph nodes (LNs) contributes to functional improvement. High endothelial venules (HEVs) are specialized vessels that allow lymphocytes to enter LNs. In this study, we compared the numbers of HEVs and lymphocytes in LNs after vascularized and nonvascularized LNT. Methods Fifty mice were divided into three groups (group 1, pedicled vascularized LNT; group 2, pedicled nonvascularized LNT; group 3, free nonvascularized LNT). Afferent lymphatic reconnection was confirmed by patent blue staining. The transferred LNs were harvested 4 weeks after surgery. HEVs, B-cells, and T-cells were subjected to immunohistochemical staining and quantified. Results Afferent lymphatic reconnection was observed in 13 of 20 transferred LNs in group 1, 11 of 15 in group 2, and 7 of 15 in group 3. The ratio of dilated/total HEVs in transferred LNs with afferent lymphatic reconnection was significantly higher in group 1 than in groups 2 and 3. No significant differences in numbers of B-cells and T-cells were found in the transferred LNs. Conclusions We found that more functional HEVs were preserved in cases with successful afferent lymphatic reconnection after vascularized LNT than after nonvascularized LNT.