Comparative interactome analysis reveals distinct and overlapping properties of Raf family kinases
Comparative interactome analysis reveals distinct and overlapping properties of Raf family kinases
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比较相互作用组分析揭示了 Raf 家族激酶的独特且重叠的特性
DOI：
10.1016/j.bbrc.2019.05.089
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发表时间：
2019
影响因子：
3.1
通讯作者：
Xu Tian-Rui
中科院分区：
文献类型：
--
作者：
Zhang Xin-Yu;Guo Hui;Huang Ying;Hao Pei-Qi;Yang Yang;Liu Ying;Guo Xiao-Xi;Hao Qian;An Su;Xu Tian-Rui
三种哺乳动物Raf蛋白（A-Raf、B-Raf和C-Raf）是MAPK途径的关键组分。虽然不同的功能已提出Raf激酶，它仍然不清楚如何相互作用的蛋白质有助于三个Raf激酶的信号功能的差异。在这里，我们报告的比较相互作用组的三个Raf激酶血清饥饿和EGF刺激的条件下。我们确定了近400种新的相互作用蛋白质;一些与所有三种亚型相互作用，而另一些只与一种或两种相互作用。在不同条件下比较三种Raf激酶的相互作用组揭示Raf蛋白通过特异性相互作用执行不同的功能。我们的相互作用组数据有助于定义三种Raf激酶之间的差异，并可能揭示新的功能或调控机制。Raf激酶蛋白质-蛋白质相互作用的知识将有助于我们在未来研究特定途径的功能。
The three mammalian Raf proteins (A-Raf, B-Raf, and C-Raf) are key components of the MAPK pathway. Although diverse functions have been proposed for Raf kinases, it is still not clear how interacting proteins contribute to differences in the signaling functions of the three Raf kinases. Here, we report the comparative interactomes of the three Raf kinases under serum-starved and EGF-stimulated conditions. We identified nearly 400 novel interacting proteins; some interacted with all three isoforms while others interacted exclusively with one or two. Comparing the interactomes of the three Raf kinases under different conditions revealed Raf proteins perform distinct functions through specific interactions. Our interactome data help define the differences between the three Raf kinases and may uncover new functions or regulatory mechanisms. Knowledge of Raf kinase protein-protein interactions will help us to investigate the function of specific pathways in the future.