Long-term Effects on the Histology and Function of Livers and Spleens in Rats after 33% Toploading of PEG-PLA-nano Artificial Red Blood Cells
Long-term Effects on the Histology and Function of Livers and Spleens in Rats after 33% Toploading of PEG-PLA-nano Artificial Red Blood Cells
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长期%20效果%20on%20the%20组织学%20and%20功能%20of%20肝脏%20and%20脾%20in%20大鼠%20after%2033%%20Toploading%20of%20PEG-PLA-nano%20Artificial%20Red%20Blood%20Cells
DOI：
10.1080/10731190802554224
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
T. Chang
中科院分区：
文献类型：
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作者：
Z. Liu;T. Chang
本研究旨在探讨含有血红蛋白和红细胞酶的纳米尺寸PEG-PLA人工红细胞在1/3血容量顶部负荷后对大鼠肝脏和脾脏的长期影响。实验大鼠接受以下输注之一：乳酸林格液中的纳米人造红细胞、乳酸林格液、无基质血红蛋白、多血红蛋白和自体大鼠全血。在输注前以及输注后第1、7和21天采集血样进行分析。纳米人工红细胞、多聚血红蛋白、乳酸林格和大鼠红细胞对丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、肌酸激酶、淀粉酶和肌酸激酶均无明显不良影响。另一方面，无基质血红蛋白对肝脏产生显着的不利影响，如 21 天内丙氨酸转氨酶和天冬氨酸转氨酶升高所示。输注后第21天，处死大鼠并切除肝脏和脾脏进行组织学检查。纳米人工红细胞、多聚血红蛋白、乳酸林格氏细胞和大鼠红细胞均未引起肝脏和脾脏的显微组织学异常。在无基质血红蛋白组中，肝脏显示血红蛋白在中央静脉和血窦中积聚，以及肝脂肪变性。总之，注射的纳米人工红细胞可以被网状内皮系统有效代谢和清除，并且不会对肝脏或脾脏产生任何生化或组织学不良影响。
This study is to investigate the long-term effects of nanodimension PEG-PLA artificial red blood cells containing hemoglobin and red blood cell enzymes on the liver and spleen after 1/3 blood volume top loading in rats. The experimental rats received one of the following infusions: Nano artificial red blood cells in Ringer lactate, Ringer lactate, stroma-free hemoglobin, polyhemoglobin, and autologous rat whole blood. Blood samples were taken before infusions and on days 1, 7, and 21 after infusions for analysis. Nano artificial red blood cells, polyhemoglobin, Ringer lactate and rat red blood cells did not have any significant adverse effects on alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase, amylase and creatine kinase. On the other hand, stroma-free hemoglobin induced significant adverse effects on liver as shown by elevation in alanine aminotransferase and aspartate aminotransferase throughout the 21 days. On day 21 after infusions rats were sacrificed and livers and spleens were excised for histological examination. Nano artificial red blood cells, polyhemoglobin, Ringer lactate and rat red blood cells did not cause any abnormalities in the microscopic histology of the livers and spleens. In the stroma-free hemoglobin group the livers showed accumulation of hemoglobin in central veins and sinusoids, and hepatic steatosis. In conclusion, injected nano artificial red blood cells can be efficiently metabolized and removed by the reticuloendothelial system, and do not have any biochemical or histological adverse effects on the livers or the spleens.