OsDGD2β is the Sole Digalactosyldiacylglycerol Synthase Gene Highly Expressed in Anther, and its Mutation Confers Male Sterility in Rice
OsDGD2β is the Sole Digalactosyldiacylglycerol Synthase Gene Highly Expressed in Anther, and its Mutation Confers Male Sterility in Rice
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DOI：
10.1186/s12284-019-0320-z
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发表时间：
2019-08-14
期刊：
影响因子：
5.5
通讯作者：
Shu, Qingyao
中科院分区：
文献类型：
--
作者：
Basnet, Rasbin;Hussain, Nazim;Shu, Qingyao
背景二半乳糖基二酰基甘油（DGDG）是主要存在于蓝细菌、真核藻类和高等植物的光合膜中的主要脂质之一。 DGDG 与 MGDG（单半乳糖基二酰基甘油）一起形成叶绿体类囊体膜中的基质，为含氧光合作用的光化学和电子传递反应提供场所。结果 计算机分析显示水稻（Oryza sativa L.）基因组中有5个编码DGDG合酶的基因，这些基因在不同组织中差异表达，其中OsDGD2β被确定为唯一在花药中表达的DGDG合酶基因。然后，我们利用 CRISPR/Cas9 系统开发了 osdgd2 beta 突变体，并阐明了其作用，特别是在花药和花粉的发育中。 OsDGD2β功能的丧失导致水稻雄性不育，其特征是花药呈淡黄色且皱缩，花粉中缺乏淀粉颗粒，并且绒毡层细胞退化延迟。 osdgd2 beta中花药中的总脂肪酸和DGDG含量分别降低了18.66%和22.72%，证实了DGDG在花药发育中的重要性。突变体在营养表型上没有显着差异，叶子中 DGDG 合酶基因的相对基因表达、叶绿素测量和光合参数分析证实了这一点，这意味着 OsDGD2 beta 在花药中的特异性。结论 总的来说，我们证明了 DGDG 在花粉发育中的重要性以及 OsDGD2 beta 功能丧失导致的雄性不育。在这里，我们还提出将 OsDGD2 beta 在利用核雄性不育系统的杂交水稻育种中的应用。
Background Digalactosyldiacylglycerol (DGDG) is one of the major lipids found predominantly in the photosynthetic membrane of cyanobacteria, eukaryotic algae and higher plants. DGDG, along with MGDG (Monogalactosyldiacylglycerol), forms the matrix in thylakoid membrane of chloroplast, providing the site for photochemical and electron transport reactions of oxygenic photosynthesis. Results In silico analysis reveals that rice (Oryza sativa L.) genome has 5 genes encoding DGDG synthase, which are differentially expressed in different tissues, and OsDGD2 beta was identified to be the sole DGDG synthase gene expressed in anther. We then developed osdgd2 beta mutants by using the CRISPR/Cas9 system and elucidate its role, especially in the development of anther and pollen. The loss of function of OsDGD2 beta resulted in male sterility in rice characterized by pale yellow and shrunken anther, devoid of starch granules in pollen, and delayed degeneration of tapetal cells. The total fatty acid and DGDG content in the anther was reduced by 18.66% and 22.72% in osdgd2 beta, affirming the importance of DGDG in the development of anther. The mutants had no notable differences in the vegetative phenotype, as corroborated by relative gene expression of DGDG synthase genes in leaves, chlorophyll measurements, and analysis of photosynthetic parameters, implying the specificity of OsDGD2 beta in anther. Conclusion Overall, we showed the importance of DGDG in pollen development and loss of function of OsDGD2 beta results in male sterility. Here, we have also proposed the use of OsDGD2 beta in hybrid rice breeding using the nuclear male sterility system.