Binding of human and animal immunoglobulins to the IgG Fe receptor induced by human cytomegalovirus
Binding of human and animal immunoglobulins to the IgG Fe receptor induced by human cytomegalovirus
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DOI：
10.1099/0022-1317-82-5-1137
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发表时间：
2001-05-01
影响因子：
3.8
通讯作者：
Johansson, PJH
中科院分区：
文献类型：
--
作者：
Antonsson, A;Johansson, PJH
人巨细胞病毒（HCMV）感染的细胞表达一种病毒编码的受体，该受体能够结合IgG的Fc部分，已经检测了这种Fc受体（FcR）的一些基本结合特性。人IgG Fc片段与丙酮固定、HCMV感染的人胚肺成纤维细胞相互作用的亲和常数（K-a）估计约为2 x 10（8）M-1，结合位点数估计约为2 x 10（6）/细胞。在人IgG、伊加、IgM和IgD类别中，仅IgG与受体反应，并且所有四种IgG亚类都是反应性的。来自兔、仓鼠、猫、猪和马的IgG表现出与HCMV FcR的结合，而来自小鼠、大鼠、豚鼠、狗、绵羊、山羊、牛和鸡的IgG则相反。具有和不具有HCMV IgG FcR结合特性的免疫球蛋白，如来自兔和小鼠的IgG，可以在揭示受体的功能重要性方面具有价值。当针对单纯疱疹病毒1型诱导的FcR测试免疫球蛋白时，相对于HCMV FcR，观察到免疫反应性的相似性和差异，这使得IgG分子上这两种疱疹病毒FcR的结合位点不太可能相同。
Human cytomegalovirus (HCMV)-infected cells express a virus-encoded receptor that is able to bind the Fc part of IgG, Some basic binding properties of this Fc receptor (FcR) have been examined. The affinity constant (K-a) for human IgG Fc fragment in its interaction with acetone-fixed, HCMV-infected human embryonic lung fibroblasts was estimated to be around 2 x 10(8) M-1 and the number of binding sites was estimated to be around 2 x 10(6) per cell. Of the human IgG, IgA, IgM and IgD classes, only IgG reacted with the receptor, and all four of the IgG subclasses were reactive. IgG from rabbit, hamster, cat, swine and horse exhibited binding to the HCMV FcR, in contrast to IgG from mouse, rat, guinea pig, dog, sheep, goat, cow and chicken, Immunoglobulins with and without HCMV IgG FcR-binding properties, like IgG from rabbit and mouse, can be of value in revealing the functional importance of the receptor. When the immunoglobulins were tested against herpes simplex virus type 1-induced FcR, both similarities and differences in immunoreactivity were seen relative to the HCMV FcR, which makes it unlikely that the binding sites for these two herpesvirus FcRs on the IgG molecule are identical.