α-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin.
α-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin.
复制标题
α-Synemin 通过与 titin 的 M10 区域相互作用定位到肌节的 M 带。
DOI：
10.1016/j.febslet.2014.11.001
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发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
Russell,MaryA
中科院分区：
文献类型：
--
作者：
Prudner,BethanyC;Roy,PritamSinha;Damron,DerekS;Russell,MaryA
α-Synemin contains a unique 312 amino acid insert near the end of its C-terminal tail. Therefore we set out to determine if the insert is a site of protein–protein interaction that regulates the sub-cellular localization of this large isoform of synemin. Yeast-two hybrid analysis indicated that this region is a binding site for the M10 region of titin. This was confirmed with GST pull-down assays. Co-immunoprecipitation of endogenous proteins indicated close association of the two proteins in vivo and immunostaining of cardiomyocytes demonstrated co-localization of the proteins at the M-band of the sarcomere.
