Enterotype Bacteroides Is Associated with a High Risk in Patients with Diabetes: A Pilot Study
Enterotype Bacteroides Is Associated with a High Risk in Patients with Diabetes: A Pilot Study
复制标题
肠型拟杆菌与糖尿病患者的高风险相关：一项初步研究
DOI：
10.1155/2020/6047145
复制
发表时间：
2020-01-22
影响因子：
4.3
通讯作者：
Chen, Li
中科院分区：
文献类型：
--
作者：
Wang, Jiajia;Li, Wenjuan;Chen, Li
越来越多的研究关注胃肠道微生物组与2型糖尿病之间的关系，但很少有人真正探讨肠道菌群与2型糖尿病之间的关系。材料和方法。我们招募了134名2型糖尿病患者和37名非糖尿病对照。测量每个受试者的人体测量和临床指标。还收集了每个受试者的粪便样品，并进行16 S rDNA测序。采用多元Logistic回归分析确定肠型与2型糖尿病的关系。在调整年龄、BMI、TG、HDL-C、DAO和TNF-α后，采用多元线性回归分析探讨脂多糖水平与胰岛素敏感性的关系。采用斯皮尔曼相关分析法对各因子与菌群进行相关性分析。采用偏相关分析确定各因素之间的相关性。结果2型糖尿病组肠道菌群多样性低于非糖尿病对照组。所有受试者的粪便群落聚成两个肠型，以类杆菌和普雷沃菌的水平区分。Logistic回归分析显示，拟杆菌属和肠型拟杆菌属与普雷沃氏菌属。偏相关分析表明，脂多糖与二胺氧化酶，肿瘤坏死因子-α和Gutt胰岛素敏感性指数密切相关，调整多个协变量后。此外，脂多糖水平被认为是胰岛素敏感性的独立危险因素。结论我们确定了两种肠型，拟杆菌和普雷沃菌，在所有的科目。我们的研究结果表明，肠型拟杆菌是2型糖尿病的独立危险因素，这是由于脂多糖水平升高导致胰岛素敏感性降低。偏相关分析表明，脂多糖与二胺氧化酶，肿瘤坏死因子-α和Gutt胰岛素敏感性指数密切相关，调整多个协变量后。此外，脂多糖水平被认为是胰岛素敏感性的独立危险因素。拟杆菌和
Background More and more studies focus on the relationship between the gastrointestinal microbiome and type 2 diabetes, but few of them have actually explored the relationship between enterotypes and type 2 diabetes. Materials and Methods. We enrolled 134 patients with type 2 diabetes and 37 nondiabetic controls. The anthropometric and clinical indices of each subject were measured. Fecal samples of each subject were also collected and were processed for 16S rDNA sequencing. Multiple logistic regression analysis was used to determine the associations of enterotypes with type 2 diabetes. Multiple linear regression analysis was used to explore the relationship between lipopolysaccharide levels and insulin sensitivity after adjusting for age, BMI, TG, HDL-C, DAO, and TNF-α. The correlation analysis between factors and microbiota was identified using Spearman correlation analysis. The correlation analysis between factors was identified using partial correlation analysis. Results Gut microbiota in type 2 diabetes group exhibited lower bacterial diversity compared with nondiabetic controls. The fecal communities from all subjects clustered into two enterotypes distinguished by the levels of Bacteroides and Prevotella. Logistic regression analysis showed that the Bacteroides and Bacteroides and Prevotella enterotype. Partial correlation analysis showed that lipopolysaccharide was closely associated with diamine oxidase, tumor necrosis factor-alpha, and Gutt insulin sensitivity index after adjusting for multiple covariates. Furthermore, the level of lipopolysaccharide was found to be an independent risk factor for insulin sensitivity. Conclusions We identified two enterotypes, Bacteroides and Prevotella, among all subjects. Our results showed that the Bacteroides enterotype was an independent risk factor for type 2 diabetes, which was due to increased levels of lipopolysaccharide causing decreased insulin sensitivity.Bacteroides and Prevotella enterotype. Partial correlation analysis showed that lipopolysaccharide was closely associated with diamine oxidase, tumor necrosis factor-alpha, and Gutt insulin sensitivity index after adjusting for multiple covariates. Furthermore, the level of lipopolysaccharide was found to be an independent risk factor for insulin sensitivity. Bacteroides and