Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns.
Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns.
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DOI：
10.1016/j.bbrc.2010.05.055
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发表时间：
2010-06-18
影响因子：
3.1
通讯作者：
Lee, Byeong Jae
中科院分区：
文献类型：
--
作者：
Kim, Jin Young;Lee, Kwang Hee;Shim, Myoung Sup;Shin, Hyein;Xu, Xue-Ming;Carlson, Bradley A.;Hatfield, Dolph L.;Lee, Byeong Jae
硒磷酸合成酶1（Selenophosphate synthetase 1，SPS 1）是高等真核生物的一个必需基因。鉴定了人SPS 1的五种可变剪接变体（主要类型，ΔE2、ΔE8、+E9、+E9 a），其中+E9和+E9 a产生相同的蛋白质。主要分布在核膜和质膜上，其余分布在细胞质中。所有变异体都形成同源二聚体，此外，主要类型与ΔE2形成异源二聚体，ΔE8与+E9形成异源二聚体。每种剪接变体的表达水平在不同的细胞系中是不同的。每个可变剪接变体的表达在细胞周期中受到调节。主要型和ΔE8水平逐渐升高，至G2/M期后逐渐下降。ΔE2表达在S期中期达到高峰，随后逐渐下降。细胞周期阻滞后，+E9/+ E9 a表达逐渐下降。SPS 1剪接变异体在细胞周期调控的可能参与进行了讨论。
Selenophosphate synthetase 1 (SPS1) is an essential cellular gene in higher eukaryotes. Five alternative splice variants of human SPS1 (major type, ΔE2, ΔE8, +E9, +E9a) were identified wherein +E9 and +E9a make the same protein. The major type was localized in both the nuclear and plasma membranes, and the others in the cytoplasm. All variants form homodimers, and in addition, the major type forms a heterodimer with ΔE2, and ΔE8 with +E9. The level of expression of each splice variant was different in various cell lines. The expression of each alternative splice variant was regulated during the cell cycle. The levels of the major type and ΔE8 were gradually increased until G2/M phase and then gradually decreased. ΔE2 expression peaked at mid-S phase and then gradually decreased. However, +E9/+E9a expression decreased gradually after cell cycle arrest. The possible involvement of SPS1 splice variants in cell cycle regulation is discussed.
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DOI：
10.1073/pnas.87.2.543
发表时间：
1990-01-01
影响因子：
11.1
作者：
LEINFELDER, W;FORCHHAMMER, K;BOCK, A
通讯作者：
BOCK, A
影响因子：
5.3
作者：
Small-Howard, A;Morozova, N;Berry, MJ
通讯作者：
Berry, MJ
影响因子：
5.6
作者：
Wang, Kai-Tuo;Wang, Juan;Su, Xiao-Dong
通讯作者：
Su, Xiao-Dong
影响因子：
4
作者：
Morey, M;Corominas, M;Serras, F
通讯作者：
Serras, F
影响因子：
4.8
作者：
LOW, SC;HARNEY, JW;BERRY, MJ
通讯作者：
BERRY, MJ