Analysis of the human HP1 interactome reveals novel binding partners
Analysis of the human HP1 interactome reveals novel binding partners
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DOI：
10.1016/j.bbrc.2011.08.059
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发表时间：
2011-09-23
影响因子：
3.1
通讯作者：
Angrand, Pierre-Olivier
中科院分区：
文献类型：
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作者：
Rosnoblet, Claire;Vandamme, Julien;Angrand, Pierre-Olivier
异染色质蛋白1（Heterochromatin protein 1，HP 1）是果蝇中一种重要的组成型异染色质，是表观遗传基因沉默的重要组成部分。关于三种哺乳动物HP 1同种型CBX 1、CBX 3和CBX 5的了解较少。在这里，我们采用了串联亲和纯化的方法，再加上串联质谱方法，以确定相互作用的合作伙伴的哺乳动物HP 1同种型。我们的分析以高置信度鉴定了约30-40种与CBX 1和CBX 3共洗脱的蛋白质，以及约10种与CBX 5共洗脱的蛋白质，包括许多新的HP 1结合伴侣。我们的数据还表明，HP 1家族成员主要与一个单一的合作伙伴，或在小的蛋白质复合物组成的有限数量的组件。最后，我们发现，轻微的结合偏好可能存在于HP 1家族成员之间。(C)2011 Elsevier Inc. All rights reserved.
Heterochromatin protein 1 (HP1) has first been described in Drosophila as an essential component of constitutive heterochromatin required for stable epigenetic gene silencing. Less is known about the three mammalian HP1 isotypes CBX1, CBX3 and CBX5. Here, we applied a tandem affinity purification approach coupled with tandem mass spectrometry methodologies in order to identify interacting partners of the mammalian HP1 isotypes. Our analysis identified with high confidence about 30-40 proteins co-eluted with CBX1 and CBX3, and around 10 with CBX5 including a number of novel HP1-binding partners. Our data also suggest that HP1 family members are mainly associated with a single partner or within small protein complexes composed of limited numbers of components. Finally, we showed that slight binding preferences might exist between HP1 family members. (C) 2011 Elsevier Inc. All rights reserved.