Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis
Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis
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DOI：
10.1002/dvg.20428
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发表时间：
2008-09-01
期刊：
影响因子：
1.5
通讯作者：
Ko, CheMyong
中科院分区：
文献类型：
--
作者：
Bridges, Phillip J.;Koo, Yongburn;Ko, CheMyong
一种在细胞色素P-450 17 α-羟化酶/17，20-裂解酶（Cyp 17）启动子调控下表达iCre的转基因小鼠系被开发为一种新的转基因小鼠模型，用于特异性地在卵巢的卵泡膜/间质细胞和睾丸的Leydig细胞中进行基因的条件性缺失。在这份报告中，我们描述了Cyp 17 iCre小鼠的发展和应用这些小鼠的雌激素受体α（Esr 1）基因的有条件删除在卵泡膜/间质和间质细胞的女性和男性性腺，分别。这些小鼠将被证明是一种强有力的工具，以细胞特异性的方式在性腺中复制基因。
A transgenic mouse line that expresses iCre under regulation of the Cytochrome P-450 17 alpha-hydroxylase/17, 20-lyase (Cyp17) promoter was developed as a novel transgenic mouse model for the conditional deletion of genes specifically in the theca/interstitial cells of the ovary and Leydig cells of the testis. In this report, we describe the development of Cyp17iCre mice and the application of these mice for conditional deletion of the estrogen receptor alpha (Esr1) gene in the theca/interstitial and Leydig cells of the female and male gonad, respectively. These mice will prove a powerful tool to inactivate genes in the gonad in a cell-specific manner.