Effect of epidermal growth factor and transforming growth factor β1 on growth and invasive potentials of newly established rat bladder carcinoma cell lines
Effect of epidermal growth factor and transforming growth factor β1 on growth and invasive potentials of newly established rat bladder carcinoma cell lines
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表皮生长因子和转化生长因子β1对新建大鼠膀胱细胞癌细胞系生长和侵袭能力的影响
DOI：
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发表时间：
1993
影响因子：
6.4
通讯作者：
R. Oyasu
中科院分区：
文献类型：
--
作者：
H. Kawamata;S. Kameyama;L. Nan;K. Kawai;R. Oyasu
我们建立了5个大鼠膀胱癌细胞系（MYU 3L、MYU 4、MYU 6s、MYKUIL和MYP 3）。EGF刺激DNA合成的所有细胞在单层培养，无论EGF受体的数量。在软琼脂中，只有MYU 3L形成菌落，EGF促进其生长。EGF不能诱导其他细胞在软琼脂中生长。相比之下，TGF-β1抑制细胞的生长，但致瘤细胞和从大的体内肿瘤建立的细胞比其他细胞对TGF-β1更具抗性。我们测试了生长因子对来自新建立的细胞系的MYP 3细胞（非致瘤性）、MYU 3L细胞（致瘤性/高度侵袭性但非转移性）和另一种转移性细胞系LMC 19的侵袭潜力的影响。MYP 3仅表达痕量的92 kDa明胶酶（MMP-9），而MYU 3L表达间质胶原酶（MMP-1）和MMP-9，LMC 19表达72 kDa明胶酶（MMP-2）和MMP-9。TGF-β1刺激LMC 19中MMP-2的释放，但EGF对两种细胞中任何MMP的释放均无影响。这些观察结果表明，EGF作为有丝分裂原的所有测试的细胞，但没有提高恶性表型。此外，对TGF-β1抑制作用的反应性丧失可能是恶性表型的重要一步。一些恶性肿瘤可能利用TGF-β1来增强其侵袭和转移能力。
We established 5 rat bladder cell lines (MYU3L, MYU4, MYU6s, MYKUIL and MYP3). EGF stimulated DNA synthesis of all the cells in monolayer culture, regardless of the number of EGF receptors. In soft agar, only MYU3L formed colonies, and EGF enhanced their growth. However, EGF did not induce the other cells to grow in soft agar. In contrast, TGF‐β1 inhibited the growth of the cells, but a tumorigenic cell and the cells which were established from large in vivo tumors were more resistant than the others to TGF‐β1. We tested the effect of growth factors on the invasive potential of MYP3 cells (non‐tumorigenic), MYU3L cells (tumorigenic/highly invasive but not metastatic) from newly established cell lines, and another metastatic cell line, LMC 19. MYP3 expressed only a trace amount of 92‐kDa gelatinase (MMP‐9), whereas MYU3L expressed interstitial collagenase (MMP‐1) and MMP‐9, and LMC19 expressed 72‐kDa gelatinase (MMP‐2) and MMP‐9. The release of MMP‐2 in LMC 19 was stimulated by TGF‐β1, but EGF had no effect on the release of any MMPs in either type of cells. These observations suggest that EGF acted as a mitogen on all the cells tested, but did not enhance the malignant phenotype. Further, the loss of responsiveness to the suppressive effect of TGF‐β1 may be an important step toward a malignant phenotype. Some of malignant tumors may utilize TGF‐β1 for enhancing their invasive and metastatic potential.
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人类癌细胞系对结缔组织的侵袭：对尿激酶、尿激酶受体和间质胶原酶的需求。
影响因子：
11.2
作者：
Ossowski,L
通讯作者：
Ossowski,L
尿表皮生长因子在大鼠膀胱癌发生中的促癌作用。
影响因子：
11.2
作者：
Momose,H;Kakinuma,H;Shariff,SY;Mitchell,GB;Rademaker,A;Oyasu,R
通讯作者：
Oyasu,R
影响因子：
11.2
作者：
E. Messing
通讯作者：
E. Messing
基质金属蛋白酶基因在高和低转移潜能的转化大鼠细胞系中的表达。
影响因子：
11.2
作者：
Sreenath,T;Matrisian,LM;Stetler-Stevenson,W;Gattoni-Celli,S;Pozzatti,RO
通讯作者：
Pozzatti,RO
DOI：
10.1073/pnas.87.19.7678
发表时间：
1990-10-01
影响因子：
11.1
作者：
WELCH, DR;FABRA, A;NAKAJIMA, M
通讯作者：
NAKAJIMA, M