A comparison of manual and semi-automated methods in the assessment of axonal injury.
A comparison of manual and semi-automated methods in the assessment of axonal injury.
复制标题
手动和半自动方法在轴突损伤评估中的比较。
DOI：
10.1046/j.1365-2990.1999.00159.x
复制
发表时间：
1999
影响因子：
5
通讯作者：
Graham,DI
中科院分区：
文献类型：
--
作者：
Gentleman,SM;McKenzie,JE;Royston,MC;McIntosh,TK;Graham,DI
中枢神经系统弥漫性轴索损伤（DAI）是创伤后昏迷的常见原因，可能导致不同程度的残疾，甚至包括植物人状态。人类和动物的实验研究以前依赖于半定量分级系统来确定DAI的程度与患者临床特征之间的关系。利用β -淀粉样蛋白前体免疫细胞化学方法检测15例致命性头部损伤的胼胝体切片中的DAI，我们开发了一种定量图像分析技术来评估轴索损伤。这种新方法是客观和可重复性的，并且可以更好地与生物力学、放射学和临床参数相关联，以增加我们对DAI的理解。
Diffuse axonal injury (DAI) in the central nervous system is a common cause of post‐traumatic coma and may result in varying degrees of disability up to and including the vegetative state. Experimental studies in man and animals have previously relied upon semiquantitative grading systems for determining the relationship between the extent of DAI and the clinical features of patients. Using β‐amyloid precursor protein immunocytochemistry for the detection of DAI in sections of corpus callosum from 15 cases of fatal head injury, we have developed a quantitative image analysis technique for the assessment of axonal injury. This new method is objective and reproducible and should allow better correlation with biomechanical, radiological, and clinical parameters to increase our understanding of DAI.