Reactive glial cells: increased stiffness correlates with increased intermediate filament expression
Reactive glial cells: increased stiffness correlates with increased intermediate filament expression
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反应性胶质细胞：硬度增加与中间丝表达增加相关
DOI：
10.1096/fj.10-163790
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发表时间：
2011-02-01
期刊：
影响因子：
4.8
通讯作者：
Kaes, Josef A.
中科院分区：
文献类型：
--
作者：
Lu, Yun-Bi;Iandiev, Ianors;Kaes, Josef A.
反应性神经胶质细胞的硬度增加可能会阻碍神经突的生长，并导致哺乳动物中枢神经系统的再生能力较差。我们通过缺血再灌注在啮齿动物视网膜中诱导反应性神经胶质增生，并评估了野生型小鼠、缺乏胶质纤维酸性蛋白和波形蛋白（GFAP（-/-）Vim（-/-））（其中神经胶质细胞因此缺乏IF）的小鼠和正常Long-Evans大鼠中中间丝（IF）的表达和解离的单个神经胶质细胞的粘弹性特性。针对缺血再灌注，野生型小鼠和大鼠中的神经胶质细胞显着变硬，但 GFAP(-/-)Vim(-/-) 小鼠中的神经胶质细胞没有变化。细胞硬度（弹性模量）与 IF 的密度相关。这些结果支持以下假设：刚性神经胶质疤痕会损害神经再生，并且 IF 是反应性神经胶质细胞粘弹性的重要决定因素。因此，治疗性抑制反应性神经胶质细胞中的 IF 上调可能会促进神经再生。-Lu, Y.-B.、Iandiev, I.、Hollborn, M.、Korber, N.、Ulbricht, E.、Hirrlinger, P. G.、Pannicke, T.、Wei, E.-Q.、Bringmann, A.、Wolburg, H.、Wilhelmsson, U.、Pekny, M., Wiedemann，P.，Reichenbach，A.，Kas，J.A.反应性胶质细胞：硬度增加与中间丝表达增加相关。 FASEB J. 25, 624-631 (2011)。 www.fasebj.org
Increased stiffness of reactive glial cells may impede neurite growth and contribute to the poor regenerative capabilities of the mammalian central nervous system. We induced reactive gliosis in rodent retina by ischemia-reperfusion and assessed intermediate filament (IF) expression and the viscoelastic properties of dissociated single glial cells in wild-type mice, mice lacking glial fibrillary acidic protein and vimentin (GFAP(-/-)Vim(-/-)) in which glial cells are consequently devoid of IFs, and normal Long-Evans rats. In response to ischemia-reperfusion, glial cells stiffened significantly in wild-type mice and rats but were unchanged in GFAP(-/-)Vim(-/-) mice. Cell stiffness (elastic modulus) correlated with the density of IFs. These results support the hypothesis that rigid glial scars impair nerve regeneration and that IFs are important determinants of cellular viscoelasticity in reactive glia. Thus, therapeutic suppression of IF up-regulation in reactive glial cells may facilitate neuroregeneration.-Lu, Y.-B., Iandiev, I., Hollborn, M., Korber, N., Ulbricht, E., Hirrlinger, P. G., Pannicke, T., Wei, E.-Q., Bringmann, A., Wolburg, H., Wilhelmsson, U., Pekny, M., Wiedemann, P., Reichenbach, A., Kas, J. A. Reactive glial cells: increased stiffness correlates with increased intermediate filament expression. FASEB J. 25, 624-631 (2011). www.fasebj.org