Synthesis of Peptides and Proteins with Site-Specific Glutamate Arginylation.
Synthesis of Peptides and Proteins with Site-Specific Glutamate Arginylation.
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DOI：
10.1007/978-1-0716-2942-0_22
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发表时间：
2023-01-01
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影响因子：
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通讯作者：
Petersson, E James
中科院分区：
文献类型：
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作者：
Shimogawa, Marie;Huang, Yun;Petersson, E James
固相多肽合成和蛋白质半合成是对多肽和蛋白质进行定点修饰的有效方法。我们描述了使用这些技术在特定位点合成具有谷氨酸脱乙酰化（EArg）的肽和蛋白质的协议。这些方法克服了酶促去乙酰化方法所带来的挑战，并允许全面研究EArg对蛋白质折叠和相互作用的影响。潜在的应用包括生物物理分析，基于细胞的显微镜研究，以及分析人体组织样本中的EArg水平和相互作用组。
Solid-phase peptide synthesis and protein semi-synthesis are powerful methods for site-specific modification of peptides and proteins. We describe protocols using these techniques for the syntheses of peptides and proteins bearing glutamate arginylation (EArg) at specific sites. These methods overcome challenges posed by enzymatic arginylation methods and allow for a comprehensive study of the effects of EArg on protein folding and interactions. Potential applications include biophysical analyses, cell-based microscopic studies, and profiling of EArg levels and interactomes in human tissue samples.