The soybean root-specific protein kinase GmWNK1 regulates stress-responsive ABA signaling on the root system architecture.
The soybean root-specific protein kinase GmWNK1 regulates stress-responsive ABA signaling on the root system architecture.
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DOI：
10.1111/j.1365-313x.2010.04320.x
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发表时间：
2010-10
期刊：
影响因子：
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通讯作者：
Yingxiang Wang;H. Suo;Yan Zheng;Kaidong Liu;C. Zhuang;K. Kahle;Hong Ma;Xiaolong Yan
中科院分区：
文献类型：
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作者：
Yingxiang Wang;H. Suo;Yan Zheng;Kaidong Liu;C. Zhuang;K. Kahle;Hong Ma;Xiaolong Yan
在人类中，WNK蛋白激酶家族的成员是细胞体积稳态和上皮离子转运的神经元敏感性调节剂，并且这些蛋白质的突变由于肾NaCl重吸收增加而导致罕见的遗传性高血压形式。最近在植物中发现了一类相关的激酶，但它们的功能在很大程度上是未知的。我们已经确定了根特异性WNK激酶同源物，GmWNK 1，在大豆（大豆）。GmWNK 1的表达在根中，特别是在与侧根形成相关的根细胞中被检测到，并且被脱落酸（阿坝）以及甘露醇、蔗糖、聚乙二醇和NaCl下调。体外和体内实验表明，GmWNK 1与另一种大豆蛋白GmCYP 707 A1相互作用，GmCYP 707 A1是阿坝催化剂中的关键阿坝8 '-羟化酶。此外，与野生型相比，35 S-GmWNK 1转基因大豆植株的侧根数量和长度减少，表明GmWNK 1在调节根系构型中的作用。我们认为GmWNK 1的功能是微调依赖于ABA的阿坝稳态，从而介导阿坝和渗透信号对根系结构的调节。该研究揭示了来自重要主食作物大豆的植物WNK 1基因的新功能，并确定了一种调节途径的新组分，该调节途径不仅参与阿坝信号传导，而且还参与与已知阿坝信号传导途径不同的ABA依赖性机制对侧根形成的抑制。
In humans, members of the WNK protein kinase family are osmosensitive regulators of cell volume homeostasis and epithelial ion transport, and mutation of these proteins causes a rare inherited form of hypertension due to increased renal NaCl re-absorption. A related class of kinases was recently discovered in plants, but their functions are largely unknown. We have identified a root-specific WNK kinase homolog, GmWNK1, in soybean (Glycine max). GmWNK1 expression was detected in the root, specifically in root cells associated with lateral root formation, and was down-regulated by abscisic acid (ABA), as well as by mannitol, sucrose, polyethylene glycol and NaCl. In vitro and in vivo experiments showed that GmWNK1 interacts with another soybean protein, GmCYP707A1, which is a key ABA 8'-hydroxylase that functions in ABA catabolism. Furthermore, 35S-GmWNK1 transgenic soybean plants had reduced lateral root number and length compared with wild-type, suggesting a role of GmWNK1 in the regulation of root system architecture. We propose that GmWNK1 functions to fine-tune ABA-dependent ABA homeostasis, thereby mediating the regulation of the root system architecture by ABA and osmotic signals. The study has revealed a new function of a plant WNK1 gene from the important staple crop soybean, and has identified a new component of a regulatory pathway that is involved not only in ABA signaling, but also in the repression of lateral root formation by an ABA-dependent mechanism distinct from known ABA signaling pathways.