Characterization of the humoral immune response in dogs after vaccination against the Lyme borreliosis agent -: A study with five commercial vaccines using two different vaccination schedules
Characterization of the humoral immune response in dogs after vaccination against the Lyme borreliosis agent -: A study with five commercial vaccines using two different vaccination schedules
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DOI：
10.1016/j.vaccine.2006.07.031
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发表时间：
2007-01-04
期刊：
影响因子：
5.5
通讯作者：
Straubinger, Reinhard K.
中科院分区：
文献类型：
--
作者：
Toepfer, Katharina H.;Straubinger, Reinhard K.
使用五种市售疫苗，对各组狗接种莱姆病疏螺旋体疫苗，并随访13个月。在研究中纳入了改良的疫苗接种计划，试图在免疫接种的第一年诱导更高的保护性抗体水平，从而延长保护性抗体水平。接种疫苗后，使用ELISA和Western印迹法监测抗体。血清样品用来自伯氏疏螺旋体的抗原制剂进行检查。s.，B。garinii或B。afzelii裂解物或分别与来源于三个物种的重组OspA。无论使用哪种疫苗，第三次接种均诱导显著更高的抗体水平。用同源物和异源物检测系统对血清的进一步分析显示，疫苗抗体在体外仅对源自异源疏螺旋体属物种的抗原具有较小的交叉反应性，因此仅可预期最小的交叉保护。(c)2006爱思唯尔有限公司保留所有权利。
Using five commercially available vaccines, groups of dogs were vaccinated against Lyme borreliosis and followed for 13 months. A modified vaccination schedule was included in the study in an attempt to induce higher and therefore longer-lasting protective antibody levels during the first year of immunization. Following vaccination antibodies were monitored using ELISA and Western blotting. Serum samples were examined either with antigen preparations derived from either Borrelia burgdorferi s. s., B. garinii or B. afzelii lysates or separately with recombinant OspA derived from the three species. Regardless of the vaccine used the third vaccination induced significantly higher antibody levels. Further analyses of the sera with homologue and heterologue detection systems showed in vitro only a minor cross-reactivity of vaccinal antibodies towards antigens derived from heterologous Borrelia species in vitro and consequently only minimal cross-protection can be expected. (c) 2006 Elsevier Ltd. All rights reserved.